ArticlePlanta2007
Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 85 citations in OpenAlex.
- Defensins of Grasses: A Systematic Review.Biomolecules · 2020Pooled it
- Wild genes to the rescue: high-throughput genomics reveals the wild source of broomrape resistance in sunflower.Journal of experimental botany · 2026Article
- Antifungal Peptides SmAPAntibiotics (Basel, Switzerland) · 2025Article
- Antimicrobial Plant Peptides: Structure, Classification, Mechanism and Therapeutic Potential.Current topics in medicinal chemistry · 2025Review
- Characterization and functional analysis of gerberaaBIOTECH · 2024Article
- Article
- Plant antimicrobial peptides: structures, functions, and applications.Botanical studies · 2021Review
- Peptides Derived From the α-Core and γ-Core Regions of a PutativeFrontiers in microbiology · 2021Article
- Management of Infection by Parasitic Weeds: A Review.Plants (Basel, Switzerland) · 2020Review
- Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals.Plants (Basel, Switzerland) · 2018Review
- Disease Resistance Mechanisms in Plants.Genes · 2018Review
- Use of Blue-Green Fluorescence and Thermal Imaging in the Early Detection of Sunflower Infection by the Root Parasitic WeedFrontiers in plant science · 2017Article
- Article
- Tandem combination of Trigonella foenum-graecum defensin (Tfgd2) and Raphanus sativus antifungal protein (RsAFP2) generates a more potent antifungal protein.Functional & integrative genomics · 2013Article
- Antifungal proteins: More than antimicrobials?Fungal biology reviews · 2013Article
- Tomato GDSL1 is required for cutin deposition in the fruit cuticle.The Plant cell · 2012Article
- Four plant defensins from an indigenous South African Brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes.BMC research notes · 2011Article
- Antimicrobial peptides: modes of mechanism, modulation of defense responses.Plant signaling & behavior · 2011Review
- Article
- Towards effective resistance to Striga in African maize.Plant signaling & behavior · 2008Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant defensins are small basic peptides of 5-10 kDa and most of them exhibit antifungal activity. In a sunflower resistant to broomrape, among the three defensin encoding cDNA identified, SF18, SD2 and HaDef1, only HaDef1 presented a preferential root expression pattern and was induced upon infection by the root parasitic plant Orobanche cumana. The amino acid sequence deduced from HaDef1 coding sequence was composed of an endoplasmic reticulum signal sequence of 28 amino acids, a standard defensin domain of 50 amino-acid residues and an unusual C-terminal domain of 30 amino acids with a net positive charge. A 5.8 kDa recombinant mature Ha-DEF1 corresponding to the defensin domain was produced in Escherichia coli and was purified by means of a two-step chromatography procedure, Immobilized Metal Affinity Chromatography (IMAC) and Ion Exchange Chromatography. Investigation of in vitro antifungal activity of Ha-DEF1 showed a strong inhibition on Saccharomyces cerevisiae growth linked to a membrane permeabilization, and a morphogenetic activity on Alternaria brassicicola germ tube development, as already reported for some other plant defensins. Bioassays also revealed that Ha-DEF1 rapidly induced browning symptoms at the radicle apex of Orobanche seedlings but not of another parasitic plant, Striga hermonthica, nor of Arabidopsis thaliana. FDA vital staining showed that these browning areas corresponded to dead cells. These results demonstrate for the first time a lethal effect of defensins on plant cells. The potent mode of action of defensin in Orobanche cell death and the possible involvement in sunflower resistance are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.