ArticlePloS one2007
Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 297 papers.
What it found
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Who cites it
297 citing papers in PubMed.
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
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- Advantages of human opsins in optogenetic visual restoration.Frontiers in neuroscience · 2025Review
- A structural and functional bioinformatics study of QTY-designed retinylidene proteins.QRB discovery · 2025Article
- A Prefrontal→Periaqueductal Gray Pathway Differentially Engages Autonomic, Hormonal, and Behavioral Features of the Stress-Coping Response.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Prefrontal projections to the bed nuclei of the stria terminalis modulate the specificity of aversive memories.Research square · 2024Article
- Dual color optogenetic tool enables heart arrest, bradycardic, and tachycardic pacing in Drosophila melanogaster.Communications biology · 2024Article
- Optogenetic Vision Restoration.Cold Spring Harbor perspectives in medicine · 2024Review
- Optogenetics and Targeted Gene Therapy for Retinal Diseases: Unravelling the Fundamentals, Applications, and Future Perspectives.Journal of clinical medicine · 2024Review
- Optogenetic Inhibition of Rat Anterior Cingulate Cortex Impairs the Ability to Initiate and Stay on Task.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Article
- Optogenetics: Illuminating the Future of Hearing Restoration and Understanding Auditory Perception.Current gene therapy · 2024Review
- Nanoparticle-based optical interfaces for retinal neuromodulation: a review.Frontiers in cellular neuroscience · 2024Review
- Cellular and subcellular optogenetic approaches towards neuroprotection and vision restoration.Progress in retinal and eye research · 2023Review
- Computational analysis of multichannel magnetothermal neural stimulation using magnetic resonator array.Biomedical engineering letters · 2023Article
- Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity.Scientific reports · 2023Article
- Indirect Effects of Halorhodopsin Activation: Potassium Redistribution, Nonspecific Inhibition, and Spreading Depolarization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Practical considerations in an era of multicolor optogenetics.Frontiers in cellular neuroscience · 2023Article
- Nociception in fruit fly larvae.Frontiers in pain research (Lausanne, Switzerland) · 2023Review
- Optogenetics Sheds Light on Brown and Beige Adipocytes.Journal of cellular signaling · 2023Article
237 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.
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Registered trials
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