ArticleJournal of the Royal Society, Interface2007
An integrated systems biology approach to understanding the rules of keratinocyte colony formation.
Article in Journal of the Royal Society, Interface, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- Review
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- Individualised computational modelling of immune mediated disease onset, flare and clearance in psoriasis.PLoS computational biology · 2022Article
- A computational model of the epidermis with the deformable dermis and its application to skin diseases.Scientific reports · 2021Article
- The Role of Calcium in Wound Healing.International journal of molecular sciences · 2021Review
- Role of subnetworks mediated by [Formula: see text], IL-23/IL-17 and IL-15 in a network involved in the pathogenesis of psoriasis.Scientific reports · 2021Article
- A 3D self-organizing multicellular epidermis model of barrier formation and hydration with realistic cell morphology based on EPISIM.Scientific reports · 2017Article
- Modelling epidermis homoeostasis and psoriasis pathogenesis.Journal of the Royal Society, Interface · 2015Article
- Adult Stem Cell Therapies for Wound Healing: Biomaterials and Computational Models.Frontiers in bioengineering and biotechnology · 2015Review
- ORAI1 calcium channel orchestrates skin homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Skin stem cell hypotheses and long term clone survival--explored using agent-based modelling.Scientific reports · 2013Article
- Article
- Modeling drug- and chemical-induced hepatotoxicity with systems biology approaches.Frontiers in physiology · 2012Article
- Understanding the melanocyte distribution in human epidermis: an agent-based computational model approach.PloS one · 2012Article
- Development of a three dimensional multiscale computational model of the human epidermis.PloS one · 2010Article
- Article
- Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions.Journal of materials science. Materials in medicine · 2009Article
- Multiway modeling and analysis in stem cell systems biology.BMC systems biology · 2008Article
- Agent based modelling helps in understanding the rules by which fibroblasts support keratinocyte colony formation.PloS one · 2008Article
- Intercellular adhesion: mechanisms for growth and metastasis of epithelial cancers.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Closely coupled in vitro and in virtuo models have been used to explore the self-organization of normal human keratinocytes (NHK). Although it can be observed experimentally, we lack the tools to explore many biological rules that govern NHK self-organization. An agent-based computational model was developed, based on rules derived from literature, which predicts the dynamic multicellular morphogenesis of NHK and of a keratinocyte cell line (HaCat cells) under varying extracellular Ca++ concentrations. The model enables in virtuo exploration of the relative importance of biological rules and was used to test hypotheses in virtuo which were subsequently examined in vitro. Results indicated that cell-cell and cell-substrate adhesions were critically important to NHK self-organization. In contrast, cell cycle length and the number of divisions that transit-amplifying cells could undergo proved non-critical to the final organization. Two further hypotheses, to explain the growth behaviour of HaCat cells, were explored in virtuo-an inability to differentiate and a differing sensitivity to extracellular calcium. In vitro experimentation provided some support for both hypotheses. For NHKs, the prediction was made that the position of stem cells would influence the pattern of cell migration post-wounding. This was then confirmed experimentally using a scratch wound model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.