ArticleBMC molecular biology2007
Stripped-down DNA repair in a highly reduced parasite.
Article in BMC molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
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- Adaptation to genome decay in the structure of the smallest eukaryotic ribosome.Nature communications · 2022Article
- Examination of Gene Loss in the DNA Mismatch Repair Pathway and Its Mutational Consequences in a Fungal Phylum.Genome biology and evolution · 2021Article
- Proteome size reduction in Apicomplexans is linked with loss of DNA repair and host redundant pathways.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Article
- Synergism between the Black Queen effect and the proteomic constraint on genome size reduction in the photosynthetic picoeukaryotes.Scientific reports · 2020Article
- Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes.mBio · 2019Article
- Article
- Muller's Ratchet and Ribosome Degeneration in the Obligate Intracellular ParasitesInternational journal of molecular sciences · 2018Article
- Genomic Survey of a Hyperparasitic Microsporidian Amphiamblys sp. (Metchnikovellidae).Genome biology and evolution · 2017Article
- Subtelomere organization in the genome of the microsporidian Encephalitozoon cuniculi: patterns of repeated sequences and physicochemical signatures.BMC genomics · 2016Article
- The Development of Genetic Modification Techniques in Intracellular Parasites and Potential Applications to Microsporidia.PLoS pathogens · 2015Review
- DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum.Microbiology and molecular biology reviews : MMBR · 2014Review
- DNA repair mechanisms and Toxoplasma gondii infection.Archives of microbiology · 2014Review
- Transcriptome analysis of the parasite Encephalitozoon cuniculi: an in-depth examination of pre-mRNA splicing in a reduced eukaryote.BMC genomics · 2013Article
- Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation.BMC genomics · 2013Article
- Article
- Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation.Molecular microbiology · 2011Article
- Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology.Eukaryotic cell · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEncephalitozoon cuniculi is a member of a distinctive group of single-celled parasitic eukaryotes called microsporidia, which are closely related to fungi. Some of these organisms, including E. cuniculi, also have uniquely small genomes that are within the prokaryotic range. Thus, E. cuniculi has undergone a massive genome reduction which has resulted in a loss of genes from diverse biological pathways, including those that act in DNA repair.DNA repair is essential to any living cell. A loss of these mechanisms invariably results in accumulation of mutations and/or cell death. Six major pathways of DNA repair in eukaryotes include: non-homologous end joining (NHEJ), homologous recombination repair (HRR), mismatch repair (MMR), nucleotide excision repair (NER), base excision repair (BER) and methyltransferase repair. DNA polymerases are also critical players in DNA repair processes. Given the close relationship between microsporidia and fungi, the repair mechanisms present in E. cuniculi were compared to those of the yeast Saccharomyces cerevisiae to ascertain how the process of genome reduction has affected the DNA repair pathways.
resultsE. cuniculi lacks 16 (plus another 6 potential absences) of the 56 DNA repair genes sought via BLASTP and PSI-BLAST searches. Six of 14 DNA polymerases or polymerase subunits are also absent in E. cuniculi. All of these genes are relatively well conserved within eukaryotes. The absence of genes is not distributed equally among the different repair pathways; some pathways lack only one protein, while there is a striking absence of many proteins that are components of both double strand break repair pathways. All specialized repair polymerases are also absent.
conclusionGiven the large number of DNA repair genes that are absent from the double strand break repair pathways, E. cuniculi is a prime candidate for the study of double strand break repair with minimal machinery. Strikingly, all of the double strand break repair genes that have been retained by E. cuniculi participate in other biological pathways.
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Registered trials
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