ArticleDevelopment genes and evolution2007
Ars insulator protects transgenes from long-term silencing in sea urchin larva.
Article in Development genes and evolution, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 16 citations in OpenAlex.
- Functional contribution of DCLKs in sea urchin development.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Piwi regulates Vasa accumulation during embryogenesis in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Autonomy in specification of primordial germ cells and their passive translocation in the sea urchin.Development (Cambridge, England) · 2012Article
- ISWI contributes to ArsI insulator function in development of the sea urchin.Development (Cambridge, England) · 2012Article
- Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos.Nucleic acids research · 2007Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reporter genes have been used as a powerful tool to analyze cis-regulatory elements responsible for temporal and spatial expression in the early development of sea urchin. However, here we show that the transgenes introduced into the sea urchin embryos undergo suppression in larval stage. The transgene silencing could be one of major obstacle in the analysis of regulatory regions in the late stages of development. We previously demonstrated that a DNA fragment (ArsI) located in the upstream region of sea urchin (Hemicentrotus pulcherrimus) arylsulfatase gene has the property of an insulator. We show that tandem ArsI prevents silencing of a transgene in sea urchin larvae when the ArsI is fused to the 5' end of the reporter gene. Furthermore, we demonstrate that DNA of the reporter gene introduced into the sea urchin eggs is methylated during development and that the ArsI protects the transgene from the DNA methylation.
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