ArticleCurrent genetics2007
Characterization of T-DNA insertion patterns in the genome of rice blast fungus Magnaporthe oryzae.
Article in Current genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
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- Identifying pathogenicity-related genes in the pathogenFrontiers in microbiology · 2023Article
- Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils.International journal of environmental research and public health · 2020Article
- Labeling ofGenes · 2019Article
- An efficient Agrobacterium-mediated transformation method for aflatoxin generation fungus Aspergillus flavus.Journal of microbiology (Seoul, Korea) · 2018Article
- The use of3 Biotech · 2018Article
- Embryogenic Callus as Target for Efficient Transformation ofFrontiers in plant science · 2018Article
- Low-affinity iron transport protein Uvt3277 is important for pathogenesis in the rice false smut fungus Ustilaginoidea virens.Current genetics · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Endoplasmic reticulum membrane-bound MoSec62 is involved in the suppression of rice immunity and is essential for the pathogenicity of Magnaporthe oryzae.Molecular plant pathology · 2016Article
- Rapid mapping of insertional mutations to probe cell wall regulation in Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2015Article
- A gene locus for targeted ectopic gene integration in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- Spectrum of T-DNA integrations for insertional mutagenesis of Histoplasma capsulatum.Fungal biology · 2013Article
- Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana.Molecular plant pathology · 2013Article
- Identification of virulence genes in the corn pathogen Colletotrichum graminicola by Agrobacterium tumefaciens-mediated transformation.Molecular plant pathology · 2011Article
- Article
- Agrobacterium-tumefaciens-mediated transformation of antifungal-lipopeptide-producing fungus Coleophoma empetri F-11899.Current genetics · 2009Article
- Reverse genetics through random mutagenesis in Histoplasma capsulatum.BMC microbiology · 2009Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium tumefaciens-mediated transformation (ATMT) has been proven to be a powerful strategy for gene disruption in plants and fungi. Patterns associated with transferred DNA (T-DNA) integration in plants and yeast have been studied comprehensively, whereas no detailed analysis of T-DNA integration has been reported yet in filamentous fungi. Here, we reported the T-DNA insertion patterns in the genome of filamentous fungus Magnaporthe oryzae. Using ATMT, a T-DNA tagged population consisting of 6,179 transformants of M. oryzae was constructed. With thermal asymmetric interlaced-PCR (TAIL-PCR), 623 right border (RB) flanking sequences and 124 left border (LB) flanking sequences were generated. Analysis of these flanking sequences indicated a significant integration bias toward non-coding sequences, suggesting distribution of T-DNAs was not random. Comparing to T-DNA RB, LB was nicked inaccurately and truncated frequently during integration. Chromosomal rearrangements, such as deletion, inversion, and translocation, were associated with T-DNA integration in some transformants. Our data suggest that, comparing with plant cells, T-DNA integrates into this filamentous fungus with more precise and simpler patterns. Some phenotypic mutants were observed in our T-DNA tagged population, and these transformants will be very useful for functional genomics research of M. oryzae.
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