ArticleProceedings of the National Academy of Sciences of the United States of America2007
Assess the nature of cholesterol-lipid interactions through the chemical potential of cholesterol in phosphatidylcholine bilayers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
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- Phospholipid membrane permeabilization and leakage of cell content by surfactin-CInternational microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Evaluating the impact of the membrane thickness on the function of the intramembrane protease GlpG.Biophysical journal · 2024Article
- Chemical potential measurements constrain models of cholesterol-phosphatidylcholine interactions.Biophysical journal · 2023Article
- Article
- Imaging and Manipulation of Plasma Membrane Fatty Acid Clusters Using TOF-SIMS Combined Optogenetics.Cells · 2022Article
- Rotational decoupling between the hydrophilic and hydrophobic regions in lipid membranes.Biophysical journal · 2022Article
- Membrane-Interacting DNA Nanotubes Induce Cancer Cell Death.Nanomaterials (Basel, Switzerland) · 2021Article
- Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.Molecular biology of the cell · 2021Article
- Direct Observation of Cholesterol Dimers and Tetramers in Lipid Bilayers.The journal of physical chemistry. B · 2021Article
- C-Glucosylation as a tool for the prevention of PAINS-induced membrane dipole potential alterations.Scientific reports · 2021Article
- Interleaflet Decoupling in a Lipid Bilayer at Excess Cholesterol Probed by Spectroscopic Ellipsometry and Simulations.The Journal of membrane biology · 2020Article
- Coarse-grained MD simulations reveal beta-amyloid fibrils of various sizes bind to interfacial liquid-ordered and liquid-disordered regions in phase separated lipid rafts with diverse membrane-bound conformational states.Biophysical chemistry · 2020Article
- Palmitoylated Proteins in Dendritic Spine Remodeling.Frontiers in synaptic neuroscience · 2020Article
- Curvature increases permeability of the plasma membrane for ions, water and the anti-cancer drugs cisplatin and gemcitabine.Scientific reports · 2019Article
- Pneumolysin-damaged cells benefit from non-homogeneous toxin binding to cholesterol-rich membrane domains.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- The Phase Behavior and Organization of Sphingomyelin/Cholesterol Membranes: A Deuterium NMR Study.Biophysical journal · 2018Article
- The Chemical Potential of Plasma Membrane Cholesterol: Implications for Cell Biology.Biophysical journal · 2018Article
- Neuronal Lipid Metabolism: Multiple Pathways Driving Functional Outcomes in Health and Disease.Frontiers in molecular neuroscience · 2018Review
- The influence of curvature on the properties of the plasma membrane. Insights from atomistic molecular dynamics simulations.Scientific reports · 2017Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Cholesterol plays a vital role in determining the physiochemical properties of cell membranes. However, the detailed nature of cholesterol-lipid interactions is a subject of ongoing debate. Existing conceptual models, including the Condensed Complex Model, the Superlattice Model, and the Umbrella Model, identify different molecular mechanisms as the key to cholesterol-lipid interactions. In this work, the compositional dependence of the chemical potential of cholesterol in cholesterol/phosphatidylcholine mixtures was systematically measured at high resolution at 37 degrees C by using an improved cholesterol oxidase (COD) activity assay. The chemical potential of cholesterol was found to be much higher in di18:1-PC bilayers than in di16:0-PC bilayers, indicating a more favorable interaction between cholesterol and saturated chains. More significantly, in 16:0,18:1-PC and di18:1-PC bilayers, the COD initial-reaction rate displays a series of distinct jumps near the cholesterol mole fractions (chi(C)) of 0.15, 0.25, 0.40, 0.50, and 0.57 and a peak at the cholesterol maximum solubility limit of 0.67. These jumps have been identified as the thermodynamic signatures of stable cholesterol regular distributions. In contrast, no such jumps were evident in di16:0-PC bilayers below chi(C) of 0.57. The observed chemical potential profile is in excellent agreement with previous Monte Carlo simulations based on the Umbrella Model but not with the predictions from the other models. The data further indicate that the cholesterol regular distribution domains (superlattices) are not the hypothesized condensed complexes. Those complexes were mainly implicated from studies on lipid monolayer that may not be relevant to the lipid bilayer in cell membranes.
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