ArticleProceedings of the National Academy of Sciences of the United States of America2007
Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 121 citations in OpenAlex.
- In Vitro Culture Studies for the Mitigation of Heavy Metal Stress in Plants.Plants (Basel, Switzerland) · 2023Review
- Nano-sulfides of Fe and Mn Efficiently Augmented the Growth, Antioxidant Defense System, and Metal Assimilation in Rice Seedlings.ACS omega · 2023Article
- Research progress on the biosynthesis and delivery of iron-sulfur clusters in the plastid.Plant cell reports · 2023Review
- Identification of Functional Genetic Variations Underlying Flooding Tolerance in Brazilian Soybean Genotypes.International journal of molecular sciences · 2022Article
- Fe-S Protein Synthesis in Green Algae Mitochondria.Plants (Basel, Switzerland) · 2021Review
- Integration analysis of PacBio SMRT- and Illumina RNA-seq reveals candidate genes and pathway involved in selenium metabolism in hyperaccumulator Cardamine violifolia.BMC plant biology · 2020Article
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases.Acta crystallographica. Section D, Structural biology · 2020Article
- The plastidialThe Journal of biological chemistry · 2020Article
- Direct observation of intermediates in the SufS cysteine desulfurase reaction reveals functional roles of conserved active-site residues.The Journal of biological chemistry · 2019Article
- Inter-Organelle NAD Metabolism Underpinning Light Responsive NADP Dynamics in Plants.Frontiers in plant science · 2019Review
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Assembly and Transfer of Iron-Sulfur Clusters in the Plastid.Frontiers in plant science · 2018Review
- A Program for Iron Economy during Deficiency Targets Specific Fe Proteins.Plant physiology · 2018Article
- Iron homeostasis in plants - a brief overview.Metallomics : integrated biometal science · 2017Review
- The iron-sulfur cluster biosynthesis protein SUFB is required for chlorophyll synthesis, but not phytochrome signaling.The Plant journal : for cell and molecular biology · 2017Article
- Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence.Plant physiology · 2016Article
- Frataxin Is Localized to Both the Chloroplast and Mitochondrion and Is Involved in Chloroplast Fe-S Protein Function in Arabidopsis.PloS one · 2015Article
- Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis.Plant physiology · 2013Article
- Requirements of the cytosolic iron-sulfur cluster assembly pathway in Arabidopsis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NifS-like proteins provide the sulfur (S) for the formation of iron-sulfur (Fe-S) clusters, an ancient and essential type of cofactor found in all three domains of life. Plants are known to contain two distinct NifS-like proteins, localized in the mitochondria (MtNifS) and the chloroplast (CpNifS). In the chloroplast, five different Fe-S cluster types are required in various proteins. These plastid Fe-S proteins are involved in a variety of biochemical pathways including photosynthetic electron transport and nitrogen and sulfur assimilation. In vitro, the chloroplastic cysteine desulfurase CpNifS can release elemental sulfur from cysteine for Fe-S cluster biogenesis in ferredoxin. However, because of the lack of a suitable mutant allele, the role of CpNifS has not been studied thus far in planta. To study the role of CpNifS in Fe-S cluster biogenesis in vivo, the gene was silenced by using an inducible RNAi (interference) approach. Plants with reduced CpNifS expression exhibited chlorosis, a disorganized chloroplast structure, and stunted growth and eventually became necrotic and died before seed set. Photosynthetic electron transport and carbon dioxide assimilation were severely impaired in the silenced plant lines. The silencing of CpNifS decreased the abundance of all chloroplastic Fe-S proteins tested, representing all five Fe-S cluster types. Mitochondrial Fe-S proteins and respiration were not affected, suggesting that mitochondrial and chloroplastic Fe-S assembly operate independently. These findings indicate that CpNifS is necessary for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth.
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