ArticleProceedings of the National Academy of Sciences of the United States of America2007
Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 214 citations in OpenAlex.
- Infertility treatment and offspring blood pressure-a systematic review and meta-analysis.Human reproduction update · 2025Pooled it
- Epigenetic, environmental, and sex-specific programming of long-term health in Assisted Reproductive Technologies-conceived offspring†.Biology of reproduction · 2026Review
- Melatonin promotes embryonic development by enhancing tryptophan metabolism and NAD + synthesis.BMC biology · 2026Article
- Article
- How Early-Life Programming During Embryogenesis Imprints Cellular Memory.International journal of molecular sciences · 2025Review
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- NADCommunications biology · 2025Article
- Blood pressure and lipid profiles in children born after ART with frozen embryo transfer.Human reproduction open · 2024Article
- Sex-specific effects ofJournal of molecular and cellular cardiology plus · 2023Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Effects of assisted reproductive technology on gene expression in heart and spleen tissues of adult offspring mouse.Frontiers in endocrinology · 2023Article
- Increased Arterial Responsiveness to Angiotensin II in Mice Conceived by Assisted Reproductive Technologies.International journal of molecular sciences · 2022Article
- Developmental origins of adult diseases.Medical review (2021) · 2022Review
- Parental Programming of Offspring Health: The Intricate Interplay between Diet, Environment, Reproduction and Development.Biomolecules · 2022Review
- DNA methylation profile of liver of mice conceived byJournal of developmental origins of health and disease · 2022Article
- Placental Dysfunction in Assisted Reproductive Pregnancies: Perinatal, Neonatal and Adult Life Outcomes.International journal of molecular sciences · 2022Review
- Parental obesity-induced changes in developmental programming.Frontiers in cell and developmental biology · 2022Review
- Article
- Anxiety and depression-like behaviours are more frequent in aged male mice conceived by ART compared with natural conception.Reproduction (Cambridge, England) · 2021Article
- Environmental Exposures around Conception: Developmental Pathways Leading to Lifetime Disease Risk.International journal of environmental research and public health · 2021Review
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
A key factor in the use of assisted reproductive technologies (ART) for diverse species is the safety of procedures for long-term health. By using a mouse model, we have investigated the effect of in vitro culture and embryo transfer (ET) of superovulated embryos on postnatal growth and physiological activity compared with that of embryos developing in vivo. Embryo culture from two-cell to blastocyst stages in T6 medium either with or without a protein source reduced blastocyst trophectoderm and inner cell mass cell number compared with that of embryos developing in vivo. Embryo culture and ET had minimal effects on postnatal growth when compared with in vivo development with an equivalent litter size. However, embryo culture, and to a lesser extent ET, led to an enhanced systolic blood pressure at 21 weeks compared with in vivo development independent of litter size, maternal origin, or body weight. Moreover, activity of enzymatic regulators of cardiovascular and metabolic physiology, namely, serum angiotensin-converting enzyme and the gluconeogenesis controller, hepatic phosphoenolpyruvate carboxykinase, were significantly elevated in response to embryo culture and/or ET in female offspring at 27 weeks, independent of maternal factors and postnatal growth. These animal data indicate that postnatal physiological criteria important in cardiovascular and metabolic health may be more sensitive to routine ART procedures than growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.