ArticleInfection and immunity2007
Flagella promote Escherichia coli K1 association with and invasion of human brain microvascular endothelial cells.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Effects of Yellow Light on Airborne Microbial Composition and on the Transcriptome of Typical Marker Strain in Ward.Disease markers · 2022Article
- Transcriptome profiling of avian pathogenicPeerJ · 2020Article
- Physical Extraction and Fast Protein Liquid Chromatography for Purifying Flagella Filament From UropathogenicFrontiers in cellular and infection microbiology · 2019Article
- A journey into the brain: insight into how bacterial pathogens cross blood-brain barriers.Nature reviews. Microbiology · 2017Review
- IQGAP1 Mediates Hcp1-PromotedFrontiers in cellular and infection microbiology · 2017Article
- Human Meningitis-Associated Escherichia coli.EcoSal Plus · 2016Review
- The role of H4 flagella in Escherichia coli ST131 virulence.Scientific reports · 2015Article
- The complex interplay among bacterial motility and virulence factors in different Escherichia coli infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Pathogens penetrating the central nervous system: infection pathways and the cellular and molecular mechanisms of invasion.Clinical microbiology reviews · 2014Review
- Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways.Infection and immunity · 2014Article
- Article
- Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum.Folia microbiologica · 2014Article
- Article
- Hcp family proteins secreted via the type VI secretion system coordinately regulate Escherichia coli K1 interaction with human brain microvascular endothelial cells.Infection and immunity · 2012Article
- Impairment of swimming motility by antidiarrheic Lactobacillus acidophilus strain LB retards internalization of Salmonella enterica serovar Typhimurium within human enterocyte-like cells.Antimicrobial agents and chemotherapy · 2011Article
- Extracellular loops of the Eschericia coli outer membrane protein A contribute to the pathogenesis of meningitis.The Journal of infectious diseases · 2011Article
- NlpI contributes to Escherichia coli K1 strain RS218 interaction with human brain microvascular endothelial cells.Infection and immunity · 2010Article
- The flagella of an atypical enteropathogenic Escherichia coli strain are required for efficient interaction with and stimulation of interleukin-8 production by enterocytes in vitro.Infection and immunity · 2009Article
- Transcriptome of Escherichia coli K1 bound to human brain microvascular endothelial cells.Biochemical and biophysical research communications · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli containing the K1 capsule is the leading cause of gram-negative meningitis, but the pathogenesis of this disease is not completely understood. Recent microarray experiments in which we compared the gene expression profile of E. coli K1 associated with human brain microvascular endothelial cells (HBMEC) to the gene expression profile of E. coli K1 not associated with HBMEC revealed that there was a threefold increase in the expression of the fliI gene, encoding an ATP synthase involved in flagellar synthesis and motility, in HBMEC-associated E. coli. In this study, we examined the role of flagella in E. coli K1 association with and invasion of HBMEC by constructing isogenic DeltaflhDC, DeltafliI, DeltafliC, and DeltacheW mutants that represented each class of flagellar genes. Mutations that affected the flagellum structure and flagellum formation (DeltaflhDC, DeltafliI, and DeltafliC) resulted in significant defects in motility, as well as in HBMEC association and invasion, compared to the characteristics of the wild-type strain when preparations were examined with or without centrifugation. Transcomplementation with the corresponding genes restored the levels of these mutants to the levels of the parent strain. These findings suggest that the HBMEC association and invasion defects of the mutants are most likely related to flagella and less likely due to their motility defects. This conclusion was supported by our demonstration that the cheW mutant was not motile but was able to associate with and invade HBMEC. In addition, purified recombinant flagellin reduced the association of the wild-type strain with HBMEC by approximately 40%, while it had no effect on the fliC mutant's association with HBMEC. Together, these findings indicate that flagella promote E. coli K1 binding to HBMEC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.