ArticleBiophysical journal2007
Secondary-structure analysis of denatured proteins by vacuum-ultraviolet circular dichroism spectroscopy.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 88 citations in OpenAlex.
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- Denaturation of firefly luciferase at heat shock temperatures captured in silico.Biophysical journal · 2025Article
- Interfacial Denaturation at the Droplet Simplifies the Formation of Drug-Loaded Protein Nanocapsules to Enhance Immune Response of Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Evaluation of the relationship between color-tuning of photosynthetic excitons and thermodynamic stability of light-harvesting chromoproteins.Photosynthetica · 2023Article
- Solution characterization of the dynamic conjugative entry exclusion protein TraG.Structural dynamics (Melville, N.Y.) · 2022Article
- Optimization of Hemoglobin Encapsulation within PLGA Nanoparticles and Their Investigation as Potential Oxygen Carriers.Pharmaceutics · 2021Article
- Temperature effect on the SARS-CoV-2: A molecular dynamics study of the spike homotrimeric glycoprotein.Computational and structural biotechnology journal · 2021Article
- Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study.International journal of molecular sciences · 2020Article
- Sensitive Label-Free Thermal Stability Assay for Protein Denaturation and Protein-Ligand Interaction Studies.Analytical chemistry · 2020Article
- Synchrotron-radiation vacuum-ultraviolet circular dichroism spectroscopy in structural biology: an overview.Biophysics and physicobiology · 2019Review
- G-quadruplex binding ability of TLS/FUS depends on the β-spiral structure of the RGG domain.Nucleic acids research · 2018Article
- High temperature limit of photosynthetic excitons.Nature communications · 2018Article
- Influence of solubilization and AD-mutations on stability and structure of human presenilins.Scientific reports · 2017Article
- Effects of Excipient Interactions on the State of the Freeze-Concentrate and Protein Stability.Pharmaceutical research · 2017Article
- Thioredoxin fusion construct enables high-yield production of soluble, active matrix metalloproteinase-8 (MMP-8) in Escherichia coli.Protein expression and purification · 2016Article
- Binding of glutathione and melatonin to pepsin occurs via different binding mechanisms.European biophysics journal : EBJ · 2016Article
- Non-native Conformers of Cystic Fibrosis Transmembrane Conductance Regulator NBD1 Are Recognized by Hsp27 and Conjugated to SUMO-2 for Degradation.The Journal of biological chemistry · 2016Article
- Role of Species-Specific Primary Structure Differences in Aβ42 Assembly and Neurotoxicity.ACS chemical neuroscience · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the structure of denatured proteins, we measured the vacuum-ultraviolet circular dichroism (VUVCD) spectra from 260 to 172 nm of three proteins (metmyoglobin, staphylococcal nuclease, and thioredoxin) in the native and the acid-, cold-, and heat-denatured states, using a synchrotron-radiation VUVCD spectrophotometer. The circular dichroism spectra of proteins fully unfolded by guanidine hydrochloride (GdnHCl) were also measured down to 197 nm for comparison. These denatured proteins exhibited characteristic VUVCD spectra that reflected a considerable amount of residual secondary structures. The contents of alpha-helices, beta-strands, turns, poly-L-proline type II (PPII), and unordered structures were estimated for each denatured state of the three proteins using the SELCON3 program with Protein Data Bank data and the VUVCD spectra of 31 reference proteins reported in our previous study. Based on these contents, the characteristics of the four types of denaturation were discussed for each protein. In all types of denaturation, a decrease in alpha-helices was accompanied by increases in beta-strands, PPII, and unordered structures. About 20% beta-strands were present even in the proteins fully unfolded by GdnHCl in which beta-sheets should be broken. From these results, we propose that denatured proteins constitute an ensemble of residual alpha-helices and beta-sheets, partly unfolded (or distorted) alpha-helices and beta-strands, PPII, and unordered structures.
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