ArticleBiophysical journal2007
Multidimensional detection and analysis of Ca2+ sparks in cardiac myocytes.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Assaying sarcoplasmic reticulum CaBiophysics reports · 2024Article
- SparkMaster 2: A New Software for Automatic Analysis of Calcium Spark Data.Circulation research · 2023Article
- PunctaSpecks: A tool for automated detection, tracking, and analysis of multiple types of fluorescently labeled biomolecules.Cell calcium · 2020Article
- CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells.Biophysical journal · 2018Article
- Automated fabrication of photopatterned gelatin hydrogels for organ-on-chips applications.Biofabrication · 2018Article
- Stabilization of diastolic calcium signal via calcium pump regulation of complex local calcium releases and transient decay in a computational model of cardiac pacemaker cell with individual release channels.PLoS computational biology · 2017Article
- Automated detection and analysis of depolarization events in human cardiomyocytes using MaDEC.Computers in biology and medicine · 2016Article
- High speed sCMOS-based oblique plane microscopy applied to the study of calcium dynamics in cardiac myocytes.Journal of biophotonics · 2016Article
- Systematic Characterization of Dynamic Parameters of Intracellular Calcium Signals.Frontiers in physiology · 2016Article
- An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.Cell calcium · 2014Article
- Automated detection and analysis of Ca(2+) sparks in x-y image stacks using a thresholding algorithm implemented within the open-source image analysis platform ImageJ.Biophysical journal · 2014Article
- Article
- Tracking stars: automated two-dimensional analysis of Ca²⁺ events. Focus on "Automated region of interest analysis of dynamic Ca²⁺ signals in image sequences".American journal of physiology. Cell physiology · 2012Article
- Hierarchical architecture influences calcium dynamics in engineered cardiac muscle.Experimental biology and medicine (Maywood, N.J.) · 2011Article
- Automated detection of intercellular signaling in astrocyte networks using the converging squares algorithm.Journal of neuroscience methods · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Examining calcium spark morphology and its relationship to the structure of the cardiac myocyte offers a direct means of understanding excitation-contraction coupling mechanisms. Traditional confocal line scanning achieves excellent temporal spark resolution but at the cost of spatial information in the perpendicular dimension. To address this, we developed a methodology to identify and analyze sparks obtained via two-dimensional confocal or charge-coupled device microscopy. The technique consists of nonlinearly subtracting the background fluorescence, thresholding the data on the basis of noise level, and then localizing the spark peaks via a generalized extrema test, while taking care to detect and separate adjacent peaks. In this article, we describe the algorithm, compare its performance to a previously validated spark detection algorithm, and demonstrate it by applying it to both a synthetic replica and an experimental preparation of a two-dimensional isotropic myocyte monolayer exhibiting sparks during a calcium transient. We find that our multidimensional algorithm provides better sensitivity than the conventional method under conditions of temporally heterogeneous background fluorescence, and the inclusion of peak segmentation reduces false negative rates when spark density is high. Our algorithm is robust and can be effectively used with different imaging modalities and allows spark identification and quantification in subcellular, cellular, and tissue preparations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.