ArticleBiophysical journal2007
An enquiry into metabolite domains.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Lactate-induced metabolic signaling is the potential mechanism for reshaping the brain function - role of physical exercise.Frontiers in endocrinology · 2025Review
- Local Attraction of Substrates and Co-Substrates Enhances Weak Acid and Base Transmembrane Transport.Biomolecules · 2022Review
- Proton Transport in Cancer Cells: The Role of Carbonic Anhydrases.International journal of molecular sciences · 2021Review
- Fluid Brain Glycolysis: Limits, Speed, Location, Moonlighting, and the Fates of Glycogen and Lactate.Neurochemical research · 2020Review
- Chromatin as a key consumer in the metabolite economy.Nature chemical biology · 2020Review
- Cutting off the fuel supply to calcium pumps in pancreatic cancer cells: role of pyruvate kinase-M2 (PKM2).British journal of cancer · 2020Article
- Another Consequence of the Warburg Effect? Metabolic Regulation of NaFrontiers in oncology · 2020Review
- The Astrocyte: Powerhouse and Recycling Center.Cold Spring Harbor perspectives in biology · 2015Review
- In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner.Nucleic acids research · 2014Article
- Small is fast: astrocytic glucose and lactate metabolism at cellular resolution.Frontiers in cellular neuroscience · 2013Article
- NMDA receptor-dependent afterdepolarizations are curtailed by carbonic anhydrase 14: regulation of a short-term postsynaptic potentiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Aspects of astrocyte energy metabolism, amino acid neurotransmitter homoeostasis and metabolic compartmentation.ASN neuro · 2012Review
- Compartmentation of membrane processes and nucleotide dynamics in diffusion-restricted cardiac cell microenvironment.Journal of molecular and cellular cardiology · 2012Review
- Determinants of brain cell metabolic phenotypes and energy substrate utilization unraveled with a modeling approach.PLoS computational biology · 2012Article
- Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange.The Journal of physiology · 2011Article
- Application of the principles of systems biology and Wiener's cybernetics for analysis of regulation of energy fluxes in muscle cells in vivo.International journal of molecular sciences · 2010Review
- General requirement for harvesting antennae at ca and h channels and transporters.Frontiers in neuroenergetics · 2010Article
- Towards single-cell real-time imaging of energy metabolism in the brain.Frontiers in neuroenergetics · 2010Article
- Direct measurement of energy fluxes from mitochondria into cytoplasm in permeabilized cardiac cells in situ: some evidence for Mitochondrial Interactosome.Journal of bioenergetics and biomembranes · 2009Article
- Philosophical basis and some historical aspects of systems biology: from Hegel to Noble - applications for bioenergetic research.International journal of molecular sciences · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is currently assumed that two or more pools of the same metabolite can coexist in the cytosolic compartment of mammalian cells. These pools are thought to be generated by the differential subcellular location of enzymes and transporters, much in the way calcium microdomains arise by the combined workings of channels, buffers, and pumps. With the aim of estimating the amplitude and spatial dimensions of these metabolite pools, we developed an analytical tool based on Brownian diffusion and the turnover numbers of the proteins involved. The outcome of the analysis is that ATP, glucose, pyruvate, lactate, and glutamate cannot be concentrated at their sources to an extent that would affect their downstream targets. For these metabolites, and others produced by slow enzymes or transporters and present at micromolar concentrations or higher, the cytosol behaves as a well-mixed, homogenous compartment. In contrast, the analysis showed microdomains known to be generated by calcium channels and revealed that calcium and pH nanodomains are to be found in the vicinity of slow enzymes and transporters in the steady state. The analysis can be readily applied to any other molecule, provided knowledge is available about rate of production, average concentration, and diffusion coefficient. Our main conclusion is that the notion of cytosolic compartmentation of metabolites needs reevaluation, as it seems to be in conflict with the underlying physical chemistry.
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Registered trials
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