ArticleBiophysical journal2007
Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Mechanisms of ligand recognition and channel opening for P2X2 receptors in lipid nanodiscs.Science advances · 2026Article
- Mechanisms of ligand recognition and channel opening for P2X2 receptors in lipid nanodiscs.bioRxiv : the preprint server for biology · 2025Article
- Reducing agents facilitate membrane patch seal integrity and longevity.Channels (Austin, Tex.) · 2024Article
- Membrane-coated glass electrodes for stable, low-noise electrophysiology recordings in Drosophila central neurons.Journal of neuroscience methods · 2024Article
- Protocol for detecting plastic changes in defined neuronal populations in neuropathic mice.STAR protocols · 2021Article
- An Improved Transwell Design for Microelectrode Ion-Flux Measurements.Micromachines · 2021Article
- Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes.Pflugers Archiv : European journal of physiology · 2021Review
- Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film.Journal of visualized experiments : JoVE · 2016Article
- Gigaseal mechanics: creep of the gigaseal under the action of pressure, adhesion, and voltage.The journal of physical chemistry. B · 2014Article
- Gd3+ and calcium sensitive, sodium leak currents are features of weak membrane-glass seals in patch clamp recordings.PloS one · 2014Article
- Subtype-specific control of P2X receptor channel signaling by ATP and Mg2+.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Nuclear patch-clamp electrophysiology of Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Dielectric analysis and multi-cell electrofusion of the yeast Pichia pastoris for electrophysiological studies.The Journal of membrane biology · 2012Article
- MscS-Like10 is a stretch-activated ion channel from Arabidopsis thaliana with a preference for anions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mg(2+) binding to open and closed states can activate BK channels provided that the voltage sensors are elevated.The Journal of general physiology · 2011Article
- A pressure-polishing set-up to fabricate patch pipettes that seal on virtually any membrane, yielding low access resistance and efficient intracellular perfusion.European biophysics journal : EBJ · 2011Article
- Lipid bilayer mechanics in a pipette with glass-bilayer adhesion.Biophysical journal · 2011Article
- From understanding cellular function to novel drug discovery: the role of planar patch-clamp array chip technology.Frontiers in pharmacology · 2011Article
- The receptor subunits generating NMDA receptor mediated currents in oligodendrocytes.The Journal of physiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Patch-clamp recording has revolutionized the study of ion channels, transporters, and the electrical activity of small cells. Vital to this method is formation of a tight seal between glass recording pipette and cell membrane. To better understand seal formation and improve practical application of this technique, we examine the effects of divalent ions, protons, ionic strength, and membrane proteins on adhesion of membrane to glass and on seal resistance using both patch-clamp recording and atomic force microscopy. We find that H(+), Ca(2+), and Mg(2+) increase adhesion force between glass and membrane (lipid and cellular), decrease the time required to form a tight seal, and increase seal resistance. In the absence of H(+) (10(-10) M) and divalent cations (<10(-8) M), adhesion forces are greatly reduced and tight seals are not formed. H(+) (10(-7) M) promotes seal formation in the absence of divalent cations. A positive correlation between adhesion force and seal formation indicates that high resistance seals are associated with increased adhesion between membrane and glass. A similar ionic dependence of the adhesion of lipid membranes and cell membranes to glass indicates that lipid membranes without proteins are sufficient for the action of ions on adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.