ArticleProceedings of the National Academy of Sciences of the United States of America2007
A role for a complex between activated G protein-coupled receptors in yeast cellular mating.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- A peroxidase-derived ligand that inducesFrontiers in cellular and infection microbiology · 2023Article
- Article
- Pheromone Guidance of Polarity Site Movement in Yeast.Biomolecules · 2022Article
- A Paradigm for Peptide Hormone-GPCR Analyses.Molecules (Basel, Switzerland) · 2020Review
- Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat.Scientific reports · 2020Article
- Dynamic roles for the N-terminus of the yeast G protein-coupled receptor Ste2p.Biochimica et biophysica acta. Biomembranes · 2017Article
- The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation.Biochimica et biophysica acta · 2016Article
- Quantification of mutation-derived bias for alternate mating functionalities of the Saccharomyces cerevisiae Ste2p pheromone receptor.Journal of biochemistry · 2016Article
- Structural characterization of triple transmembrane domain containing fragments of a yeast G protein-coupled receptor in an organic : aqueous environment by solution-state NMR spectroscopy.Journal of peptide science : an official publication of the European Peptide Society · 2015Article
- Mate and fuse: how yeast cells do it.Open biology · 2013Review
- Ontology integration to identify protein complex in protein interaction networks.Proteome science · 2011Article
- Yeast dynamically modify their environment to achieve better mating efficiency.Science signaling · 2011Article
- Basidiomycete mating type genes and pheromone signaling.Eukaryotic cell · 2010Review
- A constitutively active GPCR governs morphogenic transitions in Cryptococcus neoformans.The EMBO journal · 2009Article
- Structure of a double transmembrane fragment of a G-protein-coupled receptor in micelles.Biophysical journal · 2009Article
- Identification of a minimal cre1 promoter sequence promoting glucose-dependent gene expression in the beta-lactam producer Acremonium chrysogenum.Current genetics · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-cell fusion is a fundamental process that facilitates a wide variety of biological events in organisms ranging from yeast to humans. However, relatively little is actually understood with respect to fusion mechanisms. In the model organism Saccharomyces cerevisiae, mating of opposite-type cells is triggered by pheromone activation of the G protein-coupled receptors, alpha-factor receptor (Ste2p) and a-factor receptor (Ste3p), leading to mitogen-activated protein kinase signaling, growth arrest, and cellular fusion events. Herein we now provide evidence of a role for these receptors in the later cell fusion stage of mating. In vitro assays demonstrated the ability of the receptors to promote mixing of proteoliposomes containing phosphatidylserine, potentially based on a pheromone-dependent interaction between Ste2p and Ste3p that was confirmed by tandem affinity purification and cellular pull-down assays. The cellular mating activity of Ste2p was subsequently probed in vivo. Notably, a receptor-null yeast strain expressing N-terminally truncated Ste2p yielded a phenotype demonstrating wild-type signaling but arrested mating. The arrested prezygotes showed evidence of some cell wall erosion but no membrane juxtaposition at the fusion site. Further, in vitro analyses correlated this mutation with loss of the interaction between Ste2p and Ste3p and inhibition of related lipid mixing. Overall, these results support a role for a complex between activated yeast pheromone receptors in later cell fusion stages of mating, possibly mediating events at the level of cell wall digestion and membrane juxtaposition before membrane fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.