ArticleProceedings of the National Academy of Sciences of the United States of America2007
Tracking melanosomes inside a cell to study molecular motors and their interaction.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 103 citations in OpenAlex.
- Brain-Derived Neurotrophic Factor Trafficking via the Regulated Secretory Pathway: Mechanisms and Disease Implications.Molecular neurobiology · 2025Review
- Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments.The Journal of cell biology · 2025Article
- How energy determines spatial localisation and copy number of molecules in neurons.Nature communications · 2025Article
- Vimentin intermediate filaments provide structural stability to the mammalian Golgi complex.Journal of cell science · 2023Article
- Mitochondrial cellular organization and shape fluctuations are differentially modulated by cytoskeletal networks.Scientific reports · 2023Article
- Cilia proteins getting to work - how do they commute from the cytoplasm to the base of cilia?Journal of cell science · 2022Review
- Impact of N-Terminal Tags on De Novo Vimentin Intermediate Filament Assembly.International journal of molecular sciences · 2022Article
- Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites.Frontiers in physics · 2022Article
- Lipid-Specific Labeling of Enveloped Viruses with Quantum Dots for Single-Virus Tracking.mBio · 2020Article
- Spatial Cytoskeleton Organization Supports Targeted Intracellular Transport.Biophysical journal · 2018Article
- KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis.Scientific reports · 2017Article
- Transport of fungal RAB11 secretory vesicles involves myosin-5, dynein/dynactin/p25, and kinesin-1 and is independent of kinesin-3.Molecular biology of the cell · 2017Article
- Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER.Science (New York, N.Y.) · 2016Article
- Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments.Methods in enzymology · 2016Article
- Fluorescence imaging with one-nanometer accuracy (FIONA).Journal of visualized experiments : JoVE · 2014Article
- Single-molecule fluorescence and in vivo optical traps: how multiple dyneins and kinesins interact.Chemical reviews · 2014Review
- Caspase cleavage of GFAP produces an assembly-compromised proteolytic fragment that promotes filament aggregation.ASN neuro · 2013Article
- Foot-and-mouth disease virus modulates cellular vimentin for virus survival.Journal of virology · 2013Article
- KIF1Bβ transports dendritically localized mRNPs in neurons and is recruited to synapses in an activity-dependent manner.Cellular and molecular life sciences : CMLS · 2013Article
- Expanding horizons: ciliary proteins reach beyond cilia.Annual review of genetics · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Cells known as melanophores contain melanosomes, which are membrane organelles filled with melanin, a dark, nonfluorescent pigment. Melanophores aggregate or disperse their melanosomes when the host needs to change its color in response to the environment (e.g., camouflage or social interactions). Melanosome transport in cultured Xenopus melanophores is mediated by myosin V, heterotrimeric kinesin-2, and cytoplasmic dynein. Here, we describe a technique for tracking individual motors of each type, both individually and in their interaction, with high spatial (approximately 2 nm) and temporal (approximately 1 msec) localization accuracy. This method enabled us to observe (i) stepwise movement of kinesin-2 with an average step size of 8 nm; (ii) smoother melanosome transport (with fewer pauses), in the absence of intermediate filaments (IFs); and (iii) motors of actin filaments and microtubules working on the same cargo nearly simultaneously, indicating that a diffusive step is not needed between the two systems of transport. In concert with our previous report, our results also show that dynein-driven retrograde movement occurs in 8-nm steps. Furthermore, previous studies have shown that melanosomes carried by myosin V move 35 nm in a stepwise fashion in which the step rise-times can be as long as 80 msec. We observed 35-nm myosin V steps in melanophores containing no IFs. We find that myosin V steps occur faster in the absence of IFs, indicating that the IF network physically hinders organelle transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.