ArticleApplied and environmental microbiology2007
Stable isotope probing with 15N2 reveals novel noncultivated diazotrophs in soil.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 165 citations in OpenAlex.
- Key role of moss in supplementing nitrogen for plant growth under warming in a permafrost ecosystem.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A genomic view of Earth's biomes.Nature reviews. Genetics · 2026Review
- Microbiome matters: how transplantation methods and donor origins shape the successful restoration of the seagrass Posidonia oceanica.Environmental microbiome · 2025Article
- Microbial Diversity of Soil in a Mediterranean Biodiversity Hotspot: Parque Nacional La Campana, Chile.Microorganisms · 2024Article
- Effects of Reductive Soil Disinfestation Combined with Liquid-Readily Decomposable Compounds and Solid Plant Residues on the Bacterial Community and Functional Composition.Microbial ecology · 2023Article
- HT-SIP: a semi-automated stable isotope probing pipeline identifies cross-kingdom interactions in the hyphosphere of arbuscular mycorrhizal fungi.Microbiome · 2022Article
- Influence of Rosaceous Species and Driving Factors on Differentiation of Rhizospheric Bacteria in a Deciduous Broad-Leaved Forest.Current microbiology · 2022Article
- Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline.Microorganisms · 2022Article
- Limited effect of thermal pruning on wild blueberry crop and its root-associated microbiota.Frontiers in plant science · 2022Article
- Long-term continuous mono-cropping ofFrontiers in microbiology · 2022Article
- Evidence for phylogenetically and catabolically diverse active diazotrophs in deep-sea sediment.The ISME journal · 2020Article
- Simulating metagenomic stable isotope probing datasets with MetaSIPSim.BMC bioinformatics · 2020Article
- Assessment of DiazotrophicFrontiers in microbiology · 2020Article
- Elevated level of arsenic negatively influences nifH gene expression of isolated soil bacteria in culture condition as well as soil system.Environmental geochemistry and health · 2019Article
- Impacts of Arsenic and Antimony Co-Contamination on Sedimentary Microbial Communities in Rivers with Different Pollution Gradients.Microbial ecology · 2019Article
- Spatial heterogeneity of belowground microbial communities linked to peatland microhabitats with different plant dominants.FEMS microbiology ecology · 2019Article
- Reconditioning Degraded Mine Site Soils With Exogenous Soil Microbes: Plant Fitness and Soil Microbiome Outcomes.Frontiers in microbiology · 2019Article
- Diazotroph Diversity and Nitrogen Fixation in Summer Active Perennial Grasses in a Mediterranean Region Agricultural Soil.Frontiers in molecular biosciences · 2019Article
- Interspecific Plant Interactions Reflected in Soil Bacterial Community Structure and Nitrogen Cycling in Primary Succession.Frontiers in microbiology · 2018Article
- Application of stable-isotope labelling techniques for the detection of active diazotrophs.Environmental microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biological nitrogen fixation is a fundamental component of the nitrogen cycle and is the dominant natural process through which fixed nitrogen is made available to the biosphere. While the process of nitrogen fixation has been studied extensively with a limited set of cultivated isolates, examinations of nifH gene diversity in natural systems reveal the existence of a wide range of noncultivated diazotrophs. These noncultivated diazotrophs remain uncharacterized, as do their contributions to nitrogen fixation in natural systems. We have employed a novel 15N2-DNA stable isotope probing (5N2-DNA-SIP) method to identify free-living diazotrophs in soil that are responsible for nitrogen fixation in situ. Analyses of 16S rRNA genes from 15N-labeled DNA provide evidence for nitrogen fixation by three microbial groups, one of which belongs to the Rhizobiales while the other two represent deeply divergent lineages of noncultivated bacteria within the Betaproteobacteria and Actinobacteria, respectively. Analysis of nifH genes from 15N-labeled DNA also revealed three microbial groups, one of which was associated with Alphaproteobacteria while the others were associated with two noncultivated groups that are deeply divergent within nifH cluster I. These results reveal that noncultivated free-living diazotrophs can mediate nitrogen fixation in soils and that 15N2-DNA-SIP can be used to gain access to DNA from these organisms. In addition, this research provides the first evidence for nitrogen fixation by Actinobacteria outside of the order Actinomycetales.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.