ArticleJournal of bacteriology2007
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
- IscR-tmRNA regulatory axis plays a key role in multiple stress response and pathogenicity inVirulence · 2025Article
- Similar but Distinct-Biochemical Characterization of the Staphylococcus aureus Serine Hydrolases FphH and FphI.Proteins · 2025Article
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- Cooperative and Independent Functionality of tmRNA and SmpB inInternational journal of molecular sciences · 2025Article
- Biochemical and Cellular Characterization of the Function of Fluorophosphonate-Binding Hydrolase H (FphH) inACS infectious diseases · 2023Article
- Physiology of trans-translation deficiency in Bacillus subtilis - a comparative proteomics study.Proteomics · 2023Article
- Transcriptomic Analysis Reveals the Role of tmRNA on Biofilm Formation inMicroorganisms · 2022Article
- Ribosome Rescue Pathways in Bacteria.Frontiers in microbiology · 2021Review
- Internal Promoters and Their Effects on the Transcription of Operon Genes for Epothilone Production inFrontiers in bioengineering and biotechnology · 2021Article
- Cbl upregulatesPeerJ · 2021Article
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- Article
- Bacterial ribonucleases and their roles in RNA metabolism.Critical reviews in biochemistry and molecular biology · 2019Review
- Trans-translation is essential in the human pathogen Legionella pneumophila.Scientific reports · 2016Article
- Human Cells Require Non-stop Ribosome Rescue Activity in Mitochondria.PLoS genetics · 2016Article
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
- Comparative genomic analysis of seven Mycoplasma hyosynoviae strains.MicrobiologyOpen · 2015Article
- RNA sequencing provides evidence for functional variability between naturally co-existing Alteromonas macleodii lineages.BMC genomics · 2014Article
- Resolving nonstop translation complexes is a matter of life or death.Journal of bacteriology · 2014Review
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacillus subtilis has multiple stress response systems whose integrated action promotes growth and survival under unfavorable conditions. Here we address the function and transcriptional organization of a five-gene cluster containing ssrA, previously known to be important for growth at high temperature because of the role of its tmRNA product in rescuing stalled ribosomes. Reverse transcription-PCR experiments detected a single message for the secG-yvaK-rnr-smpB-ssrA cluster, suggesting that it constitutes an operon. However, rapid amplification of cDNA ends-PCR and lacZ fusion experiments indicated that operon transcription is complex, with at least five promoters controlling different segments of the cluster. One sigma(A)-like promoter preceded secG (P(1)), and internal sigma(A)-like promoters were found in both the rnr-smpB (P(2)) and smpB-ssrA intervals (P(3) and P(HS)). Another internal promoter lay in the secG-yvaK intercistronic region, and this activity (P(B)) was dependent on the general stress factor sigma(B). Null mutations in the four genes downstream from P(B) were tested for their effects on growth. Loss of yvaK (carboxylesterase E) or rnr (RNase R) caused no obvious phenotype. By contrast, smpB was required for growth at high temperature (52 degrees C), as anticipated if its product (a small ribosomal binding protein) is essential for tmRNA (ssrA) function. Notably, smpB and ssrA were also required for growth at low temperature (16 degrees C), a phenotype not previously associated with tmRNA activity. These results extend the known high-temperature role of ssrA and indicate that the ribosome rescue system is important at both extremes of the B. subtilis temperature range.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.