ArticleNucleic acids research2007
Entropically-driven binding of mithramycin in the minor groove of C/G-rich DNA sequences.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
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- Discrimination between G/C Binding Sites by Olivomycin A Is Determined by Kinetics of the Drug-DNA Interaction.International journal of molecular sciences · 2020Article
- Understanding the Role of the Transcription Factor Sp1 in Ovarian Cancer: from Theory to Practice.International journal of molecular sciences · 2020Review
- A phosphate-targeted dinuclear Cu(II) complex combining major groove binding and oxidative DNA cleavage.Nucleic acids research · 2018Article
- Antitumoral activity of the mithralog EC-8042 in triple negative breast cancer linked to cell cycle arrest in G2.Oncotarget · 2015Article
- Modulation of specificity protein 1 by mithramycin A as a novel therapeutic strategy for cervical cancer.Scientific reports · 2014Article
- Shape readout of AT-rich DNA by carbohydrates.Biopolymers · 2014Article
- Genome-wide modulation of gene transcription in ovarian carcinoma cells by a new mithramycin analogue.PloS one · 2014Article
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- A novel mithramycin analogue with high antitumor activity and less toxicity generated by combinatorial biosynthesis.Journal of medicinal chemistry · 2012Article
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- The chromomycin CmmA acetyltransferase: a membrane-bound enzyme as a tool for increasing structural diversity of the antitumour mithramycin.Microbial biotechnology · 2011Article
- Inhibition of Sp1-dependent transcription and antitumor activity of the new aureolic acid analogues mithramycin SDK and SK in human ovarian cancer xenografts.Gynecologic oncology · 2010Article
- Evaluation of molecular descriptors for antitumor drugs with respect to noncovalent binding to DNA and antiproliferative activity.BMC pharmacology · 2009Article
- Binding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals.BMC molecular biology · 2009Article
- Generation of new derivatives of the antitumor antibiotic mithramycin by altering the glycosylation pattern through combinatorial biosynthesis.Chembiochem : a European journal of chemical biology · 2008Article
- Studies of complex formation of olivomycin A and its derivatives with DNA.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antitumour antibiotic mithramycin A (MTA) is a DNA minor-groove binding ligand. It binds to C/G-rich tracts as a dimer that forms in the presence of divalent cations such as Mg(2+). Differential scanning calorimetry, UV thermal denaturation, isothermal titration calorimetry and competition dialysis were used, together with computations of the hydrophobic free energy of binding, to determine the thermodynamic profile of MTA binding to DNA. The results were compared to those obtained in parallel using the structurally related mithramycin SK (MSK). The binding of MTA to salmon testes DNA determined by UV melting studies (K(obs) = 1.2 (+/-0.3) x 10(5) M(-1)) is tighter than that of MSK (2.9 (+/-1.0) x 10(4) M(-1)) at 25 degrees C. Competition dialysis studies showed a tighter MTA binding to both salmon testes DNA (42% C + G) and Micrococcus lysodeikticus DNA (72% C + G). The thermodynamic analysis of binding data at 25 degrees C shows that the binding of MTA and MSK to DNA is entropically driven, dominated by the hydrophobic transfer of the antibiotics from solution to the DNA-binding site. Direct molecular recognition between MTA or MSK and DNA through hydrogen bonding and van der Waals contacts may also contribute significantly to complex formation.
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