ArticleMicrobial pathogenesis
Modulation of virulence factors in Francisella tularensis determines human macrophage responses.
Article in Microbial pathogenesis. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- Interleukin-27-dependent transcriptome signatures during neonatal sepsis.Frontiers in immunology · 2023Article
- OpiA, a Type Six Secretion System Substrate, Localizes to the Cell Pole and Plays a Role in Bacterial Growth and Viability inJournal of bacteriology · 2020Article
- A predictedVirulence · 2019Article
- Complement C3 as a Prompt for Human Macrophage Death during Infection with Francisella tularensis Strain SCHU S4.Infection and immunity · 2017Article
- Elevated interleukin-27 levels in human neonatal macrophages regulate indoleamine dioxygenase in a STAT-1 and STAT-3-dependent manner.Immunology · 2016Article
- Article
- Immunoproteomic profiling of Rickettsia parkeri and Rickettsia amblyommii.Ticks and tick-borne diseases · 2015Article
- The presence of interleukin-27 during monocyte-derived dendritic cell differentiation promotes improved antigen processing and stimulation of T cells.Immunology · 2015Article
- The contribution of the glycine cleavage system to the pathogenesis of Francisella tularensis.Microbes and infection · 2014Article
- IL-12 and IL-27 regulate the phagolysosomal pathway in mycobacteria-infected human macrophages.Cell communication and signaling : CCS · 2014Article
- Alternative sigma factor RpoN and its modulation protein YhbH are indispensable for Erwinia amylovora virulence.Molecular plant pathology · 2014Article
- Mycobacterium tuberculosis infection of human dendritic cells decreases integrin expression, adhesion and migration to chemokines.Immunology · 2014Article
- MyD88-dependent signaling prolongs survival and reduces bacterial burden during pulmonary infection with virulent Francisella tularensis.The American journal of pathology · 2013Article
- The intracellular environment of human macrophages that produce nitric oxide promotes growth of mycobacteria.Infection and immunity · 2013Article
- Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions.BMC research notes · 2013Article
- Article
- The use of resazurin as a novel antimicrobial agent against Francisella tularensis.Frontiers in cellular and infection microbiology · 2013Article
- Interferon-γ, tumor necrosis factor, and interleukin-18 cooperate to control growth of Mycobacterium tuberculosis in human macrophages.Cytokine · 2012Article
- A Francisella tularensis live vaccine strain that improves stimulation of antigen-presenting cells does not enhance vaccine efficacy.PloS one · 2012Article
- Development of tolerogenic dendritic cells and regulatory T cells favors exponential bacterial growth and survival during early respiratory tularemia.Journal of leukocyte biology · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Francisella tularensis, the causative agent of tularemia and Category A biodefense agent, is known to replicate within host macrophages, though the pathogenesis of this organism is incompletely understood. We have isolated a variant of F. tularensis live vaccine strain (LVS) based on colony morphology and its effect on macrophages. Human monocyte-derived macrophages produced more tumor necrosis factor alpha (TNFalpha), interleukin (IL)-1beta, IL-6, and IL-12 p40 following exposure to the variant, designated the activating variant (ACV). The immunoreactivity of the lipopolysaccharide (LPS) from both LVS and ACV was comparable to the previously described blue variant and was distinct from the gray variant of LVS. We found, however, the soluble protein fractions of LVS and ACV differed. Further investigation using two-dimensional gel electrophoresis demonstrated higher levels of several proteins in the parental LVS isolate. The differentially expressed proteins featured several associated with virulence in F. tularensis and other pathogens, including intracellular growth locus C (IglC), a sigma(54)-modulation protein family member (YhbH), and aconitase. ACV reverted to the LVS phenotype, indicated by low cytokine induction and high IglC expression, after growth in a chemically defined medium. These data provide evidence that the levels of virulence factors in F. tularensis are modulated based on culture conditions and that this modulation impacts host responses. This work provides a basis for investigation of Francisella virulence factor regulation and the identification of additional factors, co-regulated with IglC, that affect macrophage responses.
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