ArticleDevelopmental biology2007
Oocytes are required for the preantral granulosa cell to cumulus cell transition in mice.
Article in Developmental biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 114 citations in OpenAlex.
- Review
- DTPA disrupts development of preantral ovarian follicles in vitro.Reproduction & fertility · 2025Article
- Impact of Neutral Sphingomyelinase Inhibition on Small Extracellular Vesicle Production by Mural Granulosa Cells and In Vitro Folliculogenesis in Mice.Molecular reproduction and development · 2025Article
- Effect of Epidermal Growth Factor and 6-Dimethylaminopurine on In Vitro Maturation and Artificial Activation of Spix's Yellow-Toothed Cavy (Animals : an open access journal from MDPI · 2025Article
- Change in the ability of bovine granulosa cells to elongate transzonal projections and their transcriptome changes during follicle development.The Journal of reproduction and development · 2024Article
- Granulosa Cell-Layer Stiffening Prevents Escape of Mural Granulosa Cells from the Post-Ovulatory Follicle.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Understanding the Mechanisms of Diminished Ovarian Reserve: Insights from Genetic Variants and Regulatory Factors.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- Extracellular vesicles secreted by cumulus cells contain microRNAs that are potential regulatory factors of mouse oocyte developmental competence.Molecular human reproduction · 2024Article
- A spatiotemporal molecular atlas of the ovulating mouse ovary.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Essential Role of Granulosa Cell Glucose and Lipid Metabolism on Oocytes and the Potential Metabolic Imbalance in Polycystic Ovary Syndrome.International journal of molecular sciences · 2023Review
- Advances in Oocyte Maturation In Vivo and In Vitro in Mammals.International journal of molecular sciences · 2023Review
- Morphokinetic parameters of mouse oocyte meiotic maturation and cumulus expansion are not affected by reproductive age or ploidy status.Journal of assisted reproduction and genetics · 2023Article
- Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro.Molecular biology reports · 2022Article
- Quantitative morphokinetic parameters identify novel dynamics of oocyte meiotic maturation and cumulus expansion†.Biology of reproduction · 2022Article
- New hyaluronan-based biomatrix for 3-D follicle culture yields functionally competent oocytes.Reproductive biology and endocrinology : RB&E · 2022Article
- Integrated analysis of the expression profiles of the lncRNA-miRNA-mRNA ceRNA network in granulosa and cumulus cells from yak ovaries.BMC genomics · 2022Article
- In vitro growth of the ovarian follicle: taking stock of advances in research.JBRA assisted reproduction · 2022Review
- Stimulatory effects of TGFα in granulosa cells of bovine small antral follicles.Journal of animal science · 2022Article
- Zika Virus Infection in the Ovary Induces a Continuously Elevated Progesterone Level and Compromises Conception in Interferon Alpha/Beta Receptor-Deficient Mice.Journal of virology · 2022Article
- Effect of cumulin and super-GDF9 in standard and biphasic mouse IVM.Journal of assisted reproduction and genetics · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Preantral granulosa cells (PAGCs) differentiate into cumulus cells following antrum formation. Cumulus cells, but not PAGCs, are competent to undergo expansion. Experiments reported here tested the respective roles of both oocytes and FSH in the transition of preantral granulosa cells to cumulus cells competent to undergo expansion. PAGC-oocyte complexes were cultured with or without a low dose of FSH (0.005 IU/ml) and isolated PAGCs were cultured with or without oocytes. At the end of culture, complexes or isolated PAGCs were tested for their ability to undergo cumulus expansion and upregulate expansion transcripts in response to EGF or FSH (0.5 IU/ml). The ability to undergo expansion in response to EGF required the presence of oocytes but not FSH during the culture period. Likewise, complexes isolated from the ovaries of hypogonadal mice, which lack circulating gonadotropins, underwent expansion in response to EGF, but not FSH. In contrast, the ability to activate MAPK3/1 and MAPK14 and undergo expansion in response to FSH required prior exposure to low doses of FSH. However, these low levels (0.005 or 0.025 IU FSH/ml) suppressed expression of Slc38a3 and Amh, two transcripts highly expressed in cumulus cells, suggesting opposing effects of FSH on cumulus cell differentiation. In conclusion, the ability to undergo expansion in response to FSH requires prior exposure to FSH during development, while oocyte-derived factors alone are sufficient to promote the ability to undergo expansion in response to EGF. These results highlight the crucial role of oocytes in driving the differentiation of PAGCs into cumulus cells during the preantral to antral follicle transition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.