ArticleMethods (San Diego, Calif.)2007
NMR of membrane proteins in micelles and bilayers: the FXYD family proteins.
Article in Methods (San Diego, Calif.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Tools shaping drug discovery and development.Biophysics reviews · 2022Review
- Membrane Protein Structure Determination and Characterisation by Solution and Solid-State NMR.Biology · 2020Review
- Na+/Ca2+ exchange and Na+/K+-ATPase in the heart.The Journal of physiology · 2015Review
- Impact of phosphomimetic and non-phosphorylatable mutations of phospholemman on L-type calcium channels gating in HEK 293T cells.Journal of cellular and molecular medicine · 2015Article
- Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR.PloS one · 2014Article
- Phospholemman: a novel cardiac stress protein.Clinical and translational science · 2010Review
- Down-regulation of FXYD3 expression in human lung cancers: its mechanism and potential role in carcinogenesis.The American journal of pathology · 2009Article
- Effects of PKA phosphorylation on the conformation of the Na,K-ATPase regulatory protein FXYD1.Biochimica et biophysica acta · 2009Article
- Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Ligand binding in the conserved interhelical loop of CorA, a magnesium transporter from Mycobacterium tuberculosis.The Journal of biological chemistry · 2009Article
- Phospholemman regulates cardiac Na+/Ca2+ exchanger by interacting with the exchanger's proximal linker domain.American journal of physiology. Cell physiology · 2009Article
- Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis.BMC biotechnology · 2008Article
- Membrane orientation of the Na,K-ATPase regulatory membrane protein CHIF determined by solid-state NMR.Magnetic resonance in chemistry : MRC · 2007Article
- Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better.Biochimica et biophysica acta · 2007Review
- Solid-state NMR characterization of conformational plasticity within the transmembrane domain of the influenza A M2 proton channel.Biochimica et biophysica acta · 2007Article
- Structural similarity of a membrane protein in micelles and membranes.Journal of the American Chemical Society · 2007Article
- Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles.Biochemistry · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Determining the atomic resolution structures of membrane proteins is of particular interest in contemporary structural biology. Helical membrane proteins constitute one-third of the expressed proteins encoded in a genome, many drugs have membrane-bound proteins as their receptors, and mutations in membrane proteins result in human diseases. Although integral membrane proteins provide daunting technical challenges for all methods of protein structure determination, nuclear magnetic resonance (NMR) spectroscopy can be an extremely versatile and powerful method for determining their structures and characterizing their dynamics, in lipid environments that closely mimic the cell membranes. Once milligram amounts of isotopically labeled protein are expressed and purified, micelle samples can be prepared for solution NMR analysis, and lipid bilayer samples can be prepared for solid-state NMR analysis. The two approaches are complementary and can provide detailed structural and dynamic information. This paper describes the steps for membrane protein structure determination using solution and solid-state NMR. The methods for protein expression and purification, sample preparation and NMR experiments are described and illustrated with examples from the FXYD proteins, a family of regulatory subunits of the Na,K-ATPase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.