Trial reportClinical and vaccine immunology : CVI2007
Use of serological assays for diagnosis of hepatitis E virus genotype 1 and 3 infections in a setting of low endemicity.
Trial report in Clinical and vaccine immunology : CVI, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 111 citations in OpenAlex.
- Systematic review and meta-analysis of the seroprevalence of hepatitis E virus in the general population across non-endemic countries.PloS one · 2019Pooled it
- Laboratory Diagnosis of HEV Infection.Advances in experimental medicine and biology · 2023Review
- Genetic Evolution of Hepatitis E Virus.Advances in experimental medicine and biology · 2023Review
- Aptamer-based approaches for the detection of waterborne pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Performance Evaluation of Different Commercial Serological Kits for Diagnosis of Acute Hepatitis E Viral Infection.Polish journal of microbiology · 2020Article
- Seroprevalence of hepatitis E virus differs in Dutch and first generation migrant populations in Amsterdam, the Netherlands: a cross-sectional study.BMC infectious diseases · 2016Article
- Diagnostic Performance of Five Assays for Anti-Hepatitis E Virus IgG and IgM in a Large Cohort Study.Journal of clinical microbiology · 2016Article
- Molecular Biology and Infection of Hepatitis E Virus.Frontiers in microbiology · 2016Review
- Hepatitis E Virus.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2015Article
- Hepatitis A and E seroprevalence and associated risk factors: a community-based cross-sectional survey in rural Amazonia.BMC infectious diseases · 2014Article
- Detection of serum antibodies to hepatitis E virus in domestic pigs in Italy using a recombinant swine HEV capsid protein.BMC veterinary research · 2014Article
- Hepatitis e virus: current concepts and future perspectives.Current infectious disease reports · 2014Article
- Autochthonous Hepatitis E Virus Infection in Europe: A Matter of Concern for Public Health?Journal of clinical and translational hepatology · 2014Review
- Hepatitis E virus infection.Clinical microbiology reviews · 2014Review
- Epidemiology of zoonotic hepatitis E: a community-based surveillance study in a rural population in China.PloS one · 2014Article
- Transmission, diagnosis, and management of hepatitis E: an update.Hepatic medicine : evidence and research · 2014Review
- Epidemiology and associated risk factors of hepatitis e virus infection in plateau state, Nigeria.Virology : research and treatment · 2014Article
- Hospital-based surveillance for viral hemorrhagic fevers and hepatitides in Ghana.PLoS neglected tropical diseases · 2013Article
- Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus.Journal of veterinary science · 2013Article
- Diagnosis of hepatitis E.Nature reviews. Gastroenterology & hepatology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because of the occurrence of genotype 3 hepatitis E virus (HEV) in regions of low endemicity, it is important to validate the currently used serological assays for diagnosing infections with viruses belonging to this lineage, since these assays only use antigens derived from genotype 1 and 2 viruses. We evaluated the Genelabs enzyme-linked immunosorbent assay (ELISA) and the RecomBlot from Mikrogen for the detection of HEV-specific immunoglobulin M (IgM) and IgG under conditions of low endemicity. We compared test results of 16 patients with locally acquired genotype 3 HEV, 8 genotype 1 patients, 167 healthy controls from the general population, and 101 cases with hepatitis due to other viral causes. The measured specificities of the ELISA (98%) and the RecomBlot (97%) were comparable to those given by the manufacturer for IgM but were significantly lower for IgG (93% by ELISA and 66% by immunoblotting, versus reported values of 98% for ELISA and 95% for blotting). Antibody levels detected following infections with genotype 3 were lower than those following genotype 1 infections except for those measured in the IgM ELISA. Reactivity to the four antigens used in the immunoblot assay were analyzed and showed differences in the IgM immunoblot reactions between genotype 1 patients and genotype 3 patients. The ORF3 antigen was the most specific antigen. The specificity could be improved by a combined testing regimen with confirmation by immunoblotting of all positive ELISA results and by raising the cutoff of the IgG immunoblot assay without loss of sensitivity. We conclude that a combination of ELISA and immunoblotting is needed for acceptable specificity and sensitivity of HEV assays under conditions of low endemicity.
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