ArticleJournal of virology2007
Small interfering RNAs against the TAR RNA binding protein, TRBP, a Dicer cofactor, inhibit human immunodeficiency virus type 1 long terminal repeat expression and viral production.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Multiple Inhibitory Factors Act in the Late Phase of HIV-1 Replication: a Systematic Review of the Literature.Microbiology and molecular biology reviews : MMBR · 2018Pooled it
- Expression profiling of human milk derived exosomal microRNAs and their targets in HIV-1 infected mothers.Scientific reports · 2020Trial
- Virus meets RNAi. Symposium on antiviral applications of RNA interference.EMBO reports · 2008Trial
- Review
- Role of chromosome 1q copy number variation in hepatocellular carcinoma.World journal of hepatology · 2021Review
- Shedding Light on the Role of Extracellular Vesicles in HIV Infection and Wound Healing.Viruses · 2020Review
- HIV-1-based Virus-like Particles that Morphologically Resemble Mature, Infectious HIV-1 Virions.Viruses · 2019Article
- Expression of TARBP1 protein in human non-small-cell lung cancer and its prognostic significance.Oncology letters · 2018Article
- The therapeutic landscape of HIV-1 via genome editing.AIDS research and therapy · 2017Review
- MicroRNA miR-126-5p Enhances the Inflammatory Responses of Monocytes to Lipopolysaccharide Stimulation by Suppressing Cylindromatosis in Chronic HIV-1 Infection.Journal of virology · 2017Article
- Efficient inhibition of human immunodeficiency virus replication using novel modified microRNA-30a targeting 3'-untranslated region transcripts.Experimental and therapeutic medicine · 2016Article
- Novel RNA Duplex Locks HIV-1 in a Latent State via Chromatin-mediated Transcriptional Silencing.Molecular therapy. Nucleic acids · 2015Article
- HIV-1 RRE RNA acts as an RNA silencing suppressor by competing with TRBP-bound siRNAs.RNA biology · 2015Article
- RNA interference approaches for treatment of HIV-1 infection.Genome medicine · 2015Article
- Expression of protein TARBP1 in human hepatocellular carcinoma and its prognostic significance.International journal of clinical and experimental pathology · 2015Article
- MicroRNA Machinery Genes as Novel Biomarkers for Cancer.Frontiers in oncology · 2014Review
- The PKR activator, PACT, becomes a PKR inhibitor during HIV-1 replication.Retrovirology · 2013Article
- Review
- Contributions of microRNA dysregulation to cisplatin resistance in adenocarcinoma cells.Experimental cell research · 2013Article
- The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is now widely used for gene silencing in mammalian cells. The mechanism uses the RNA-induced silencing complex, in which Dicer, Ago2, and the human immunodeficiency virus type 1 (HIV-1) TAR RNA binding protein (TRBP) are the main components. TRBP is a protein that increases HIV-1 expression and replication by inhibition of the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. After HIV infection, TRBP could restrict the viral RNA through its activity in RNAi or could contribute more to the enhancement of viral replication. To determine which function will be predominant in the virological context, we analyzed whether the inhibition of its expression could enhance or decrease HIV replication. We have generated small interfering RNAs (siRNAs) against TRBP and found that they decrease HIV-1 long terminal repeat (LTR) basal expression 2-fold, and the LTR Tat transactivated level up to 10-fold. In the context of HIV replication, siRNAs against TRBP decrease the expression of viral genes and inhibit viral production up to fivefold. The moderate increase in PKR expression and activation indicates that it contributes partially to viral gene inhibition. The moderate decrease in micro-RNA (miRNA) biogenesis by TRBP siRNAs suggests that in the context of HIV replication, TRBP functions other than RNAi are predominant. In addition, siRNAs against Dicer decrease viral production twofold and impede miRNA biogenesis. These results suggest that, in the context of HIV replication, TRBP contributes mainly to the enhancement of virus production and that Dicer does not mediate HIV restriction by RNAi.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.