ArticleProceedings of the National Academy of Sciences of the United States of America2007
Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 150 citations in OpenAlex.
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- Genome-Wide Analysis of the SNARE Family in Cultivated Peanut (International journal of molecular sciences · 2023Article
- Diversity of retromer-mediated vesicular trafficking pathways in plants.Frontiers in plant science · 2023Review
- Relevance of the Exocyst in ArabidopsisInternational journal of molecular sciences · 2022Article
- Hydrogen sulfide reduces cell death through regulating autophagy during submergence in Arabidopsis.Plant cell reports · 2022Article
- Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- RAB GTPases and SNAREs at the trans-Golgi network in plants.Journal of plant research · 2022Review
- Coping with Abiotic Stress in Plants-An Endomembrane Trafficking Perspective.Plants (Basel, Switzerland) · 2022Review
- SNAREs Regulate Vesicle Trafficking During Root Growth and Development.Frontiers in plant science · 2022Review
- Vacuoles in Bryophytes: Properties, Biogenesis, and Evolution.Frontiers in plant science · 2022Review
- Effective methods for isolation and purification of extracellular vesicles from plants.Journal of integrative plant biology · 2021Article
- Abiotic Stress Triggers the Expression of Genes Involved in Protein Storage Vacuole and Exocyst-Mediated Routes.International journal of molecular sciences · 2021Article
- Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER.Membranes · 2021Article
- Article
- Extracellular Vesicles: Emerging Players in Plant Defense Against Pathogens.Frontiers in plant science · 2021Review
- On the discovery of an endomembrane compartment in plants.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- MTV proteins unveil ER- and microtubule-associated compartments in the plant vacuolar trafficking pathway.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Functions of the Plant Qbc SNARE SNAP25 in Cytokinesis and Biotic and Abiotic Stress Responses.Molecules and cells · 2020Article
- Completing Autophagy: Formation and Degradation of the Autophagic Body and Metabolite Salvage in Plants.International journal of molecular sciences · 2020Review
11 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein storage vacuole (PSV) is a plant-specific organelle that accumulates reserve proteins, one of the main agricultural products obtained from crops. Despite the importance of this process, the cellular machinery required for transport and accumulation of storage proteins remains largely unknown. Interfering with transport to PSVs has been shown to result in secretion of cargo. Therefore, secretion of a suitable marker could be used as an assay to identify mutants in this pathway. CLV3, a negative regulator of shoot stem cell proliferation, is an extracellular ligand that is rendered inactive when targeted to vacuoles. We devised an assay where trafficking mutants secrete engineered vacuolar CLV3 and show reduced meristems, a phenotype easily detected by visual inspection of plants. We tested this scheme in plants expressing VAC2, a fusion of CLV3 to the vacuolar sorting signal from the storage protein barley lectin. In this way, we determined that trafficking of VAC2 requires the SNARE VTI12 but not its close homologue, the conditionally redundant VTI11 protein. Furthermore, a vti12 mutant is specifically altered in transport of storage proteins, whereas a vti11 mutant is affected in transport of a lytic vacuole marker. These results demonstrate the specialization of VTI12 and VTI11 in mediating trafficking to storage and lytic vacuoles, respectively. Moreover, they validate the VAC2 secretion assay as a simple method to isolate genes that mediate trafficking to the PSV.
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