ArticleProceedings of the National Academy of Sciences of the United States of America2007
Fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers, 1 of them a synthesis that pooled it.
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Who cites it
76 citing papers in PubMed, 1 synthesis or guideline pooled it, 212 citations in OpenAlex.
- BioprospectingFrontiers in fungal biology · 2021Pooled it
- Heterologous Systems in the Functional Characterization of Proteins from Phytopathogenic Fungi: A Systematic Review.Molecular biotechnology · 2026Article
- Enantioselective Total Synthesis of (+)-Fusicoccadiene via Photocatalytic Polyene Isomerization.Journal of the American Chemical Society · 2026Article
- Cryo-EM Structure of the Cyclase Domain and Evaluation of Substrate Channeling in a Bifunctional Class II Terpene Synthase.Biochemistry · 2025Article
- Harnessing Genomics ofPlants (Basel, Switzerland) · 2025Article
- Cryo-EM Structure of the Cyclase Domain and Evaluation of Substrate Channeling in a Bifunctional Class II Terpene Synthase.bioRxiv : the preprint server for biology · 2025Article
- Structural insights into a bacterial terpene cyclase fused with haloacid Dehalogenase-like phosphatase.Chemical science · 2025Article
- Crystal structure and catalytic mechanism of drimenol synthase, an unusual bifunctional terpene cyclase-phosphatase.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structure of bifunctional variediene synthase yields unique insight on biosynthetic diterpene assembly and cyclization.Nature communications · 2025Article
- Evolution and diversification of the momilactone biosynthetic gene cluster in the genus Oryza.The New phytologist · 2025Article
- Total biosynthesis of cotylenin diterpene glycosides as 14-3-3 protein-protein interaction stabilizers.Chemical science · 2025Article
- Heterologous biosynthesis of cotylenol and concise synthesis of fusicoccane diterpenoids.Beilstein journal of organic chemistry · 2025Article
- Identifying sesterterpenoids via feature-based molecular networking and small-scale fermentation.Applied microbiology and biotechnology · 2024Article
- Engineering substrate channeling in a bifunctional terpene synthase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket.Synthetic and systems biotechnology · 2024Article
- Engineering Substrate Channeling in Assembly-Line Terpene Biosynthesis.bioRxiv : the preprint server for biology · 2024Article
- Genome mining and biosynthetic pathways of marine-derived fungal bioactive natural products.Frontiers in microbiology · 2024Review
- Methods for the preparation and analysis of the diterpene cyclase fusicoccadiene synthase.Methods in enzymology · 2024Article
- CusProSe: a customizable protein annotation software with an application to the prediction of fungal secondary metabolism genes.Scientific reports · 2023Article
- Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens.Angewandte Chemie (International ed. in English) · 2023Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusicoccins are a class of diterpene glucosides produced by the plant-pathogenic fungus Phomopsis amygdali. As modulators of 14-3-3 proteins, fusicoccins function as potent activators of plasma membrane H(+)-ATPase in plants and also exhibit unique biological activity in animal cells. Despite their well studied biological activities, no genes encoding fusicoccin biosynthetic enzymes have been identified. Cyclic diterpenes are commonly synthesized via cyclization of a C(20) precursor, geranylgeranyl diphosphate (GGDP), which is produced through condensation of the universal C(5) isoprene units dimethylallyl diphosphate and isopentenyl diphosphate by prenyltransferases. We found that (+)-fusicocca-2,10 (14)-diene, a tricyclic hydrocarbon precursor for fusicoccins, is biosynthesized from the C(5) isoprene units by an unusual multifunctional enzyme, P. amygdali fusicoccadiene synthase (PaFS), which shows both prenyltransferase and terpene cyclase activities. The functional analysis of truncated mutants and site-directed mutagenesis demonstrated that PaFS consists of two domains: a terpene cyclase domain at the N terminus and a prenyltransferase domain at the C terminus. These findings suggest that fusicoccadiene can be produced efficiently in the fungus by using the C(5) precursors, irrespective of GGDP availability. In fact, heterologous expression of PaFS alone resulted in the accumulation of fusicocca-2,10 (14)-diene in Escherichia coli cells, whereas no product was detected in E. coli cells expressing Gibberella fujikuroi ent-kaurene synthase, another fungal diterpene cyclase that also uses GGDP as a substrate but does not contain a prenyltransferase domain. Genome walking suggested that fusicoccin biosynthetic enzymes are encoded as a gene cluster near the PaFS gene.
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