ArticleProceedings of the National Academy of Sciences of the United States of America2007
Folate biofortification of tomato fruit.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Folate biofortification strategy by elicitation in kale baby leaves.Frontiers in plant science · 2026Article
- An Allele of Glutamate Formiminotransferase Triggers 5-Methyl-Tetrahydrofolate-to-MeFox Conversion and Facilitates Folate Biofortification in Maize.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Dual-omics analysis reveals B vitamin regulation and metabolism in Areca Catechu inflorescence.BMC genomics · 2025Article
- Enhanced folate content in bioengineered lettuce stably expressed under field conditions.Transgenic research · 2025Article
- Biological, dietetic and pharmacological properties of vitamin BNPJ science of food · 2025Review
- Synthetic biology and artificial intelligence in crop improvement.Plant communications · 2025Review
- Exploration of folate and its derivatives in grains of wheat with different colors.Frontiers in genetics · 2025Article
- Multi-omic analysis of the extension of broccoli quality during storage by folic acid.Journal of advanced research · 2024Article
- Adapting enzymes to improve their functionality in plants: why and how.Biochemical Society transactions · 2023Review
- Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation.Communications biology · 2023Article
- Reduced γ-glutamyl hydrolase activity likely contributes to high folate levels in Periyakulam-1 tomato.Horticulture research · 2023Article
- Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides.International journal of molecular sciences · 2022Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- Applications of Genomic Tools in Plant Breeding: Crop Biofortification.International journal of molecular sciences · 2022Review
- Comparative Transcriptome Analysis Reveals Mechanisms of Folate Accumulation in Maize Grains.International journal of molecular sciences · 2022Article
- Red light enhances folate accumulation in wheat seedlings.Journal of Zhejiang University. Science. B · 2021Article
- Weighted gene co-expression network analysis unveils gene networks regulating folate biosynthesis in maize endosperm.3 Biotech · 2021Article
- Genetically Modified Plants: Nutritious, Sustainable, yet Underrated.The Journal of nutrition · 2020Article
- Genetic and physiological regulation of folate in pak choi (Brassica rapa subsp. Chinensis) germplasm.Journal of experimental botany · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Folate deficiency leads to neural tube defects and other human diseases, and is a global health problem. Because plants are major folate sources for humans, we have sought to enhance plant folate levels (biofortification). Folates are synthesized from pteridine, p-aminobenzoate (PABA), and glutamate precursors. Previously, we increased pteridine production in tomato fruit up to 140-fold by overexpressing GTP cyclohydrolase I, the first enzyme of pteridine synthesis. This strategy increased folate levels 2-fold, but engineered fruit were PABA-depleted. We report here the engineering of fruit-specific overexpression of aminodeoxychorismate synthase, which catalyzes the first step of PABA synthesis. The resulting fruit contained an average of 19-fold more PABA than controls. When transgenic PABA- and pteridine-overproduction traits were combined by crossing, vine-ripened fruit accumulated up to 25-fold more folate than controls. Folate accumulation was almost as high (up to 15-fold) in fruit harvested green and ripened by ethylene-gassing, as occurs in commerce. The accumulated folates showed normal proportions of one-carbon forms, with 5-methyltetrahydrofolate the most abundant, but were less extensively polyglutamylated than controls. Folate concentrations in developing fruit did not change in controls, but increased continuously throughout ripening in transgenic fruit. Pteridine and PABA levels in transgenic fruit were >20-fold higher than in controls, but the pathway intermediates dihydropteroate and dihydrofolate did not accumulate, pointing to a flux constraint at the dihydropteroate synthesis step. The folate levels we achieved provide the complete adult daily requirement in less than one standard serving.
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