ArticleProceedings of the National Academy of Sciences of the United States of America2007
Direct visualization of Escherichia coli chemotaxis receptor arrays using cryo-electron microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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Who cites it
115 citing papers in PubMed, 173 citations in OpenAlex.
- Spontaneous switching in a protein signalling array reveals near-critical cooperativity.Nature physics · 2026Article
- Protein-protein interfaces in chemotaxis receptor complexes and their role in signaling.FEMS microbiology reviews · 2026Review
- Article
- Chemotaxis and Related Signaling Systems inBiomolecules · 2025Review
- The Role of the Insulin/Glucose Ratio in the Regulation of Pathogen Biofilm Formation.Biology · 2023Article
- Article
- A nonequilibrium allosteric model for receptor-kinase complexes: The role of energy dissipation in chemotaxis signaling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Recent structural advances in bacterial chemotaxis signalling.Current opinion in structural biology · 2023Review
- Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches.Biochemical Society transactions · 2022Review
- Using Atomistic Simulations to Explore the Role of Methylation and ATP in Chemotaxis Signal Transduction.ACS omega · 2022Article
- Studying bacterial chemosensory array with CryoEM.Biochemical Society transactions · 2021Review
- Molecular model of a sensor of two-component signaling system.Scientific reports · 2021Article
- Alternative Architecture of theBiomolecules · 2021Article
- Inducible intracellular membranes: molecular aspects and emerging applications.Microbial cell factories · 2020Review
- Regulation of the chemotaxis histidine kinase CheA: A structural perspective.Biochimica et biophysica acta. Biomembranes · 2020Review
- Article
- More than just a phase: the search for membraneless organelles in the bacterial cytoplasm.Current genetics · 2019Review
- Blue Light Is a Universal Signal forJournal of bacteriology · 2019Article
- Structural and Proteomic Changes in Viable but Non-culturableFrontiers in microbiology · 2019Article
- Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.Biochimica et biophysica acta. Proteins and proteomics · 2018Review
55 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Signal transduction in bacterial chemotaxis is initiated by the binding of extracellular ligands to a specialized family of methyl-accepting chemoreceptor proteins. Chemoreceptors cluster at distinct regions of the cell and form stable ternary complexes with the histidine autokinase CheA and the adapter protein CheW. Here we report the direct visualization and spatial organization of chemoreceptor arrays in intact Escherichia coli cells by using cryo-electron tomography and biochemical techniques. In wild-type cells, ternary complexes are arranged as an extended lattice, which may or may not be ordered, with significant variations in the size and specific location among cells in the same population. In the absence of CheA and CheW, chemoreceptors do not form observable clusters and are diffusely localized to the cell pole. At disproportionately high receptor levels, membrane invaginations containing nonfunctional, axially interacting receptor assemblies are formed. However, functional chemoreceptor arrays can be reestablished by increasing cellular levels of CheA and CheW. Our results demonstrate that chemotaxis in E. coli requires the presence of chemoreceptor arrays and that the formation of these arrays requires the scaffolding interactions of the signaling molecules CheA and CheW.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.