ArticleProceedings of the National Academy of Sciences of the United States of America2007
Induction of the celC operon of Clostridium thermocellum by laminaribiose.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Thermophilic site-specific recombination system for rapid insertion of heterologous DNA into the Clostridium thermocellum chromosome.Journal of industrial microbiology & biotechnology · 2024Article
- Article
- Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks inFrontiers in microbiology · 2021Article
- Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum.Microbial biotechnology · 2018Article
- LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313.Applied and environmental microbiology · 2017Article
- The LacI family protein GlyR3 co-regulates theBiotechnology for biofuels · 2017Article
- Transcriptome and Zymogram Analyses Reveal a Cellobiose-Dose Related Reciprocal Regulatory Effect on Cellulase Synthesis inFrontiers in microbiology · 2017Article
- Global repositioning of transcription start sites in a plant-fermenting bacterium.Nature communications · 2016Article
- A 1,3-1,4-β-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2.Applied and environmental microbiology · 2016Article
- Insight into Dominant Cellulolytic Bacteria from Two Biogas Digesters and Their Glycoside Hydrolase Genes.PloS one · 2015Article
- Clostridium thermocellum transcriptomic profiles after exposure to furfural or heat stress.Biotechnology for biofuels · 2013Article
- Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.Biotechnology for biofuels · 2013Article
- Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene expression.PloS one · 2013Article
- Global microarray analysis of carbohydrate use in alkaliphilic hemicellulolytic bacterium Bacillus sp. N16-5.PloS one · 2013Article
- Proteomic analysis of Clostridium thermocellum core metabolism: relative protein expression profiles and growth phase-dependent changes in protein expression.BMC microbiology · 2012Article
- Complex expression of the cellulolytic transcriptome of Saccharophagus degradans.Applied and environmental microbiology · 2011Article
- Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation.BMC microbiology · 2011Article
- Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Deletion of the Cel48S cellulase from Clostridium thermocellum.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Thermobifida fusca exoglucanase Cel6B is incompatible with the cellulosomal mode in contrast to endoglucanase Cel6A.Systems and synthetic biology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium thermocellum is an anaerobic, thermophilic, cellulolytic, and ethanogenic bacterium. It produces an extracellular multiprotein complex termed the cellulosome, which consists of >70 subunits, most of them glycosyl hydrolases. It also produces many free glycosyl hydrolases. How the organism commands such a large number of genes and proteins for biomass degradation is an intriguing yet unresolved question. We identified glyR3, which is cotranscribed with the cellulase/hemicellulase genes celC and licA, as a potential cellulase transcription regulator. The gel-shift assay (EMSA) revealed that the recombinant GlyR3 bound specifically to the celC promoter region. GlyR3 was also identified from the lysate of the lichenan-grown cells, which bound to the same sequence. DNase I footprinting and competitive EMSA showed the binding site to be an 18-bp palindromic sequence with one mismatch. The DNA-binding activity was specifically inhibited by laminaribiose, a beta-1-3 linked glucose dimer, in a dose-dependent manner. In in vitro transcription analysis, celC expression was repressed by rGlyR3 in a dose-dependent manner. The repression was relieved by laminaribiose, also in a dose-dependent manner. These results indicate that GlyR3 is a negative regulator of the celC operon consisting of celC, glyR3, and licA, and inducible by laminaribiose. Thus, the bacterium may modulate the biosynthesis of its enzyme components to optimize its activity on an available biomass substrate, in this case, beta-1-3 glucan, because both CelC and LicA are active on the substrate. The results further indicate that, despite the insolubility of the biomass substrate, regulation of the degradative enzymes can be accomplished through soluble sugars generated by the action of the enzymes.
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