ArticleProceedings of the National Academy of Sciences of the United States of America2007
S-adenosylmethionine directly inhibits binding of 30S ribosomal subunits to the SMK box translational riboswitch RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- Functional Validation of SAM Riboswitch Element A fromBiochemistry · 2024Article
- Riboswitch Distribution in the Human Gut Microbiome Reveals Common Metabolite Pathways.The journal of physical chemistry. B · 2024Article
- Transcriptional pause extension benefits the stand-by rather than catch-up Rho-dependent termination.Nucleic acids research · 2023Article
- Precise tuning of bacterial translation initiation by non-equilibrium 5'-UTR unfolding observed in single mRNAs.Nucleic acids research · 2022Article
- Transcriptional and translational S-box riboswitches differ in ligand-binding properties.The Journal of biological chemistry · 2020Article
- Article
- Observation of preQRNA biology · 2019Article
- Control and regulation of S-Adenosylmethionine biosynthesis by the regulatory β subunit and quinolone-based compounds.The FEBS journal · 2019Article
- Structure and ligand binding of the SAM-V riboswitch.Nucleic acids research · 2018Article
- RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview.Chemical reviews · 2018Review
- New tRNA contacts facilitate ligand binding in aProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Computational Methods for Modeling Aptamers and Designing Riboswitches.International journal of molecular sciences · 2017Review
- Co-Transcriptional Folding and Regulation Mechanisms of Riboswitches.Molecules (Basel, Switzerland) · 2017Review
- A magnesium-induced triplex pre-organizes the SAM-II riboswitch.PLoS computational biology · 2017Article
- T box riboswitches in Actinobacteria: translational regulation via novel tRNA interactions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- RNA-mediated regulation in Gram-positive pathogens: an overview punctuated with examples from the group A Streptococcus.Molecular microbiology · 2014Review
- Dual-acting riboswitch control of translation initiation and mRNA decay.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multiple conformations of SAM-II riboswitch detected with SAXS and NMR spectroscopy.Nucleic acids research · 2012Article
- Phylogenetic analysis and comparative genomics of purine riboswitch distribution in prokaryotes.Evolutionary bioinformatics online · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The S(MK) box is a conserved riboswitch motif found in the 5' untranslated region of metK genes [encoding S-adenosylmethionine (SAM) synthetase] in lactic acid bacteria, including Enterococcus, Streptococcus, and Lactococcus sp. Previous studies showed that this RNA element binds SAM in vitro, and SAM binding causes a structural rearrangement that sequesters the Shine-Dalgarno (SD) sequence by pairing with an anti-SD (ASD) element. A model was proposed in which SAM binding inhibits metK translation by preventing binding of the ribosome to the SD region of the mRNA. In the current work, the addition of SAM was shown to inhibit binding of 30S ribosomal subunits to S(MK) box RNA; in contrast, the addition of S-adenosylhomocysteine (SAH) had no effect. A mutant RNA, which has a disrupted SD-ASD pairing, was defective in SAM binding and showed no reduction of ribosome binding in the presence of SAM, whereas a compensatory mutation that restored SD-ASD pairing restored the response to SAM. Primer extension inhibition assays provided further evidence for SD-ASD pairing in the presence of SAM. These results strongly support the model that S(MK) box translational repression operates through occlusion of the ribosome binding site and that SAM binding requires the SD-ASD pairing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.