ArticleProceedings of the National Academy of Sciences of the United States of America2007
Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 88 citations in OpenAlex.
- Mechanism of action of the SWI/SNF family complexes.Nucleus (Austin, Tex.) · 2023Article
- Managing the Steady State Chromatin Landscape by Nucleosome Dynamics.Annual review of biochemistry · 2022Review
- Article
- Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement.eLife · 2019Article
- Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome.eLife · 2019Article
- Article
- Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex.The FEBS journal · 2017Article
- Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes.Nature reviews. Molecular cell biology · 2017Review
- While the revolution will not be crystallized, biochemistry reigns supreme.Protein science : a publication of the Protein Society · 2017Review
- Hsp90 and p23 Molecular Chaperones Control Chromatin Architecture by Maintaining the Functional Pool of the RSC Chromatin Remodeler.Molecular cell · 2016Article
- The Many Roles of BAF (mSWI/SNF) and PBAF Complexes in Cancer.Cold Spring Harbor perspectives in medicine · 2016Review
- CHD4 Is a Peripheral Component of the Nucleosome Remodeling and Deacetylase Complex.The Journal of biological chemistry · 2016Article
- Direct Measurement of the Structure of Reconstituted High-Density Lipoproteins by Cryo-EM.Biophysical journal · 2016Article
- Article
- The role of ATP-dependent machines in regulating genome topology.Current opinion in structural biology · 2016Review
- Nucleosome dynamics during chromatin remodeling in vivo.Nucleus (Austin, Tex.) · 2016Article
- From Structural Variation of Gene Molecules to Chromatin Dynamics and Transcriptional Bursting.Genes · 2015Review
- Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C.Nature communications · 2015Article
- Conformational flexibility and subunit arrangement of the modular yeast Spt-Ada-Gcn5 acetyltransferase complex.The Journal of biological chemistry · 2015Article
- Nucleosome structure and function.Chemical reviews · 2015Review
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin remodeling complexes (remodelers) are large, multisubunit macromolecular assemblies that use ATP hydrolysis to alter the structure and positioning of nucleosomes. The mechanisms proposed for remodeler action on nucleosomes are diverse, and require structural evaluation and insights. Previous reconstructions of remodelers using electron microscopy revealed interesting features, but also significant discrepancies, prompting new approaches. Here, we use the orthogonal tilt reconstruction method, which is well suited for heterogeneous samples, to provide a reconstruction of the yeast RSC (remodel the structure of chromatin) complex. Two interesting features are revealed: first, we observe a deep central cavity within RSC, displaying a remarkable surface complementarity for the nucleosome. Second, we are able to visualize two distinct RSC conformers, revealing a major conformational change in a large protein "arm," which may shift to further envelop the nucleosome. We present a model of the RSC-nucleosome complex that rationalizes the single molecule results obtained by using optical tweezers and also discuss the mechanistic implications of our structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.