ArticlePloS one2007
Tripotential differentiation of adherently expandable neural stem (NS) cells.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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Who cites it
47 citing papers in PubMed, 109 citations in OpenAlex.
- A co-culture system to study the effects of Poly I:C-activated microglia on the differentiation of murine primary neural stem cells.In vitro cellular & developmental biology. Animal · 2025Article
- Activation-derepression synergy enables a bHLH network to coordinate a signal-specific fate response.Cell reports · 2024Article
- G-quadruplex DNA structures in human stem cells and differentiation.Nature communications · 2022Article
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- Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells.Frontiers in cell and developmental biology · 2021Article
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- Reprogramming of Lipid Metabolism as a New Driving Force Behind Tauroursodeoxycholic Acid-Induced Neural Stem Cell Proliferation.Frontiers in cell and developmental biology · 2020Article
- Bio-electrosprayed human neural stem cells are viable and maintain their differentiation potential.F1000Research · 2020Article
- Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors.Stem cell reports · 2019Article
- Amyloid β Peptide Compromises Neural Stem Cell Fate by Irreversibly Disturbing Mitochondrial Oxidative State and Blocking Mitochondrial Biogenesis and Dynamics.Molecular neurobiology · 2019Article
- Neural Lineage Differentiation From Pluripotent Stem Cells to Mimic Human Brain Tissues.Frontiers in bioengineering and biotechnology · 2019Review
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- Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions.Scientific reports · 2017Article
- The Neurogenic Potential of Astrocytes Is Regulated by Inflammatory Signals.Molecular neurobiology · 2016Article
- Mediator subunit Med12 contributes to the maintenance of neural stem cell identity.BMC developmental biology · 2016Article
- Pharmacological Reprogramming of Fibroblasts into Neural Stem Cells by Signaling-Directed Transcriptional Activation.Cell stem cell · 2016Article
- Co-existence of intact stemness and priming of neural differentiation programs in mES cells lacking Trim71.Scientific reports · 2015Article
- MicroRNA-34a Modulates Neural Stem Cell Differentiation by Regulating Expression of Synaptic and Autophagic Proteins.Molecular neurobiology · 2015Article
- Article
- GluA2 is rapidly edited at the Q/R site during neural differentiation in vitro.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundA recent study has shown that pure neural stem cells can be derived from embryonic stem (ES) cells and primary brain tissue. In the presence of fibroblast growth factor 2 (FGF2) and epidermal growth factor (EGF), this population can be continuously expanded in adherent conditions. In analogy to continuously self-renewing ES cells, these cells were termed 'NS' cells (Conti et al., PLoS Biol 3: e283, 2005). While NS cells have been shown to readily generate neurons and astrocytes, their differentiation into oligodendrocytes has remained enigmatic, raising concerns as to whether they truly represent tripotential neural stem cells. METHODOLOGY/PRINCIPAL
findingsHere we provide evidence that NS cells are indeed tripotent. Upon proliferation with FGF2, platelet-derived growth factor (PDGF) and forskolin, followed by differentiation in the presence of thyroid hormone (T3) and ascorbic acid NS cells efficiently generate oligodendrocytes ( approximately 20%) alongside astrocytes ( approximately 40%) and neurons ( approximately 10%). Mature oligodendroglial differentiation was confirmed by transplantation data showing that NS cell-derived oligodendrocytes ensheath host axons in the brain of myelin-deficient rats. CONCLUSIONS/SIGNIFICANCE: In addition to delineating NS cells as a potential donor source for myelin repair, our data strongly support the view that these adherently expandable cells represent bona fide tripotential neural stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.