ArticleBMC genomics2007
Transcriptional profiling of Actinobacillus pleuropneumoniae under iron-restricted conditions.
Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
- Disruption-induced changes in syntrophic propionate and acetate oxidation: flocculation, cell proximity, and microbial activity.Biotechnology for biofuels and bioproducts · 2025Article
- Transcriptomic and proteomic profiling ofFrontiers in cellular and infection microbiology · 2025Article
- A Combinatorial Vaccine Containing Inactivated Bacterin and Subunits Provides Protection AgainstFrontiers in veterinary science · 2022Article
- Involvement of theFrontiers in veterinary science · 2022Article
- Role of aspartate ammonia-lyase in Pasteurella multocida.BMC microbiology · 2020Article
- Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance ofFrontiers in cellular and infection microbiology · 2020Article
- Transcriptomics of Haemophilus (Glässerella) parasuis serovar 5 subjected to culture conditions partially mimetic to natural infection for the search of new vaccine antigens.BMC veterinary research · 2018Article
- QseC Mediates Osmotic Stress Resistance and Biofilm Formation inFrontiers in microbiology · 2018Article
- Catecholamines promote Actinobacillus pleuropneumoniae growth by regulating iron metabolism.PloS one · 2015Article
- Changes in gene expression of Actinobacillus pleuropneumoniae in response to anaerobic stress reveal induction of central metabolism and biofilm formation.Journal of microbiology (Seoul, Korea) · 2014Article
- Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: A new insight into an old approach.Genetics and molecular biology · 2013Article
- Actinobacillus pleuropneumoniae genes expression in biofilms cultured under static conditions and in a drip-flow apparatus.BMC genomics · 2013Article
- Adhesion protein ApfA of Actinobacillus pleuropneumoniae is required for pathogenesis and is a potential target for vaccine development.Clinical and vaccine immunology : CVI · 2013Article
- The ClpP protease is required for the stress tolerance and biofilm formation in Actinobacillus pleuropneumoniae.PloS one · 2013Article
- Article
- A proteomic analysis of the regulon of the NarP two-component regulatory system response regulator in the bovine pathogen Mannheimia haemolytica A1.BMC research notes · 2011Article
- Cloning, expression, and characterization of TonB2 from Actinobacillus pleuropneumoniae and potential use as an antigenic vaccine candidate and diagnostic marker.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2011Article
- Predicting genetic traits and epitope analysis of apxIVA in Actinobacillus pleuropneumoniae.Journal of microbiology (Seoul, Korea) · 2011Article
- Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae.Proteome science · 2011Article
- Deciphering the iron response in Acinetobacter baumannii: A proteomics approach.Journal of proteomics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo better understand effects of iron restriction on Actinobacillus pleuropneumoniae and to identify new potential vaccine targets, we conducted transcript profiling studies using a DNA microarray containing all 2025 ORFs of the genome of A. pleuropneumoniae serotype 5b strain L20. This is the first study involving the use of microarray technology to monitor the transcriptome of A. pleuropneumoniae grown under iron restriction.
resultsUpon comparing growth of this pathogen in iron-sufficient versus iron-depleted medium, 210 genes were identified as being differentially expressed. Some genes (92) were identified as being up-regulated; many have confirmed or putative roles in iron acquisition, such as the genes coding for two TonB energy-transducing proteins and the hemoglobin receptor HgbA. Transcript profiling also led to identification of some new iron acquisition systems of A. pleuropneumoniae. Genes coding for a possible Yfe system (yfeABCD), implicated in the acquisition of chelated iron, were detected, as well as genes coding for a putative enterobactin-type siderophore receptor system. ORFs for homologs of the HmbR system of Neisseria meningitidis involved in iron acquisition from hemoglobin were significantly up-regulated. Down-regulated genes included many that encode proteins containing Fe-S clusters or that use heme as a cofactor. Supplementation of the culture medium with exogenous iron re-established the expression level of these genes.
conclusionWe have used transcriptional profiling to generate a list of genes showing differential expression during iron restriction. This strategy enabled us to gain a better understanding of the metabolic changes occurring in response to this stress. Many new potential iron acquisition systems were identified, and further studies will have to be conducted to establish their role during iron restriction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.