ArticleMicrobial ecology2007
Quantification of sulfate-reducing bacteria in industrial wastewater, by real-time polymerase chain reaction (PCR) using dsrA and apsA genes.
Article in Microbial ecology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 125 citations in OpenAlex.
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- Gated Nanosensor for Sulphate-Reducing Bacteria Detection.Nanomaterials (Basel, Switzerland) · 2025Article
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- Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria.Scientific reports · 2023Article
- Microbial functionalities and immobilization of environmental lead: Biogeochemical and molecular mechanisms and implications for bioremediation.Journal of hazardous materials · 2023Review
- Comparative Methods for Quantification of Sulfate-Reducing Bacteria in Environmental and Engineered Sludge Samples.Biology · 2023Article
- Time-dependent impact of co-matured manure with elemental sulfur and biochar on the soil agro-ecological properties and plant biomass.Scientific reports · 2023Article
- Effect of Digestate Modified with Amendments on Soil Health and Plant Biomass under Varying Experimental Durations.Materials (Basel, Switzerland) · 2023Article
- Differential hydrogen sulfide production by a human cohort in response to animal- and plant-based diet interventions.Clinical nutrition (Edinburgh, Scotland) · 2022Article
- Co-composting of cattle manure with biochar and elemental sulphur and its effects on manure quality, plant biomass and microbiological characteristics of post-harvest soil.Frontiers in plant science · 2022Article
- A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.Scientific data · 2020Article
- Biomethanation of Harmful Macroalgal Biomass in Leach-Bed Reactor Coupled to Anaerobic Filter: Effect of Water Regime and Filter Media.International journal of environmental research and public health · 2018Article
- Influence of COFrontiers in microbiology · 2018Article
- Article
- Persistent Hg contamination and occurrence of Hg-methylating transcript (hgcA) downstream of a chlor-alkali plant in the Olt River (Romania).Environmental science and pollution research international · 2016Article
- In Situ Microbial Community Succession on Mild Steel in Estuarine and Marine Environments: Exploring the Role of Iron-Oxidizing Bacteria.Frontiers in microbiology · 2016Article
- The Diversity of Sulfide Oxidation and Sulfate Reduction Genes Expressed by the Bacterial Communities of the Cariaco Basin, Venezuela.The open microbiology journal · 2016Article
- Diversity and degradation mechanism of an anaerobic bacterial community treating phenolic wastewater with sulfate as an electron acceptor.Environmental science and pollution research international · 2015Article
- Changes in Microbial Biofilm Communities during Colonization of Sewer Systems.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time polymerase chain reaction (PCR) is considered a highly sensitive method for the quantification of microbial organisms in environmental samples. This study was conducted to evaluate real-time PCR with SybrGreen detection as a quantification method for sulfate-reducing bacteria (SRB) in industrial wastewater produced by several chemical industries. We designed four sets of primers and developed standard curves based on genomic DNA of Desulfovibrio vulgaris from pure culture and on plasmids containing dissimilatory sulfate reductase (dsrA) or adenosine-5'-phosphosulfate reductase (apsA) genes of SRB. All the standard curves, two for dsrA and two for apsA genes, had a linear range between 0.95 x 10(2) and 9.5 x 10(6) copies/microL and between 1.2 x 10(3) and 1.2 x 10(7) copies/microL, respectively. The theoretical copy numbers of the tenfold dilutions of D. vulgaris genomic DNA were best estimated (between 2.7 to 10.5 times higher than theoretical numbers) by the standard curve with DSR1F and RH3-dsr-R primers. To mimic the effect of foreign DNA in environmental samples, serial dilutions of D. vulgaris genomic DNA were mixed with Escherichia coli chromosomal DNA (40 ng per assay). This influenced neither PCR amplification nor the quantification of target DNA. Industrial wastewater was sampled during a 15-month period and analyzed for the presence of SRB, based on dsrA gene amplification. SRB displayed a higher abundance during the summer (about 10(7)-10(8) targets mL(-1)) and lower during the winter (about 10(4)-10(5) targets mL(-1)). The results indicate that our real-time PCR approach can be used for detection of uncultured SRB and will provide valuable information related to the abundance of SRB in durable environmental samples, such as complex and saline industrial wastewaters.
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