ArticleApplied and environmental microbiology2007
A novel reductive dehalogenase, identified in a contaminated groundwater enrichment culture and in Desulfitobacterium dichloroeliminans strain DCA1, is linked to dehalogenation of 1,2-dichloroethane.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 71 citations in OpenAlex.
- Revisiting the dihaloelimination potential ofApplied and environmental microbiology · 2026Article
- 1,2-DCA biodegradation potential of an aquifer assessed in situ and in aerobic and anaerobic microcosms.Environmental microbiome · 2024Article
- Understanding the sources, function, and irreplaceable role of cobamides in organohalide-respiring bacteria.Frontiers in microbiology · 2024Review
- Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase OperonFrontiers in microbiology · 2022Article
- Biological synthesis of iron nanoparticles using hydrolysates from a waste-based biorefinery.Environmental science and pollution research international · 2020Article
- Reductive dechlorination of 1,2-dichloroethane in the presence of chloroethenes and 1,2-dichloropropane as co-contaminants.Applied microbiology and biotechnology · 2019Article
- Electron Fluxes in Biocathode Bioelectrochemical Systems Performing Dechlorination of Chlorinated Aliphatic Hydrocarbons.Frontiers in microbiology · 2018Article
- Identification of Multiple Dehalogenase Genes Involved in Tetrachloroethene-to-Ethene Dechlorination in aBioMed research international · 2017Article
- Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation.Frontiers in microbiology · 2016Review
- Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane.BioMed research international · 2015Article
- High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes.Frontiers in microbiology · 2014Article
- Functional genotyping of Sulfurospirillum spp. in mixed cultures allowed the identification of a new tetrachloroethene reductive dehalogenase.Applied and environmental microbiology · 2013Article
- Analysis of hydrocarbon-contaminated groundwater metagenomes as revealed by high-throughput sequencing.Molecular biotechnology · 2013Article
- Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Kinetics of 1,2-dichloroethane and 1,2-dibromoethane biodegradation in anaerobic enrichment cultures.Applied and environmental microbiology · 2013Article
- Dehalogenimonas spp. can Reductively Dehalogenate High Concentrations of 1,2-Dichloroethane, 1,2-Dichloropropane, and 1,1,2-Trichloroethane.AMB Express · 2012Article
- The physiological opportunism of Desulfitobacterium hafniense strain TCE1 towards organohalide respiration with tetrachloroethene.Applied and environmental microbiology · 2012Article
- Molecular techniques in the biotechnological fight against halogenated compounds in anoxic environments.Microbial biotechnology · 2012Review
- Pyrosequence analysis of unamplified and whole genome amplified DNA from hydrocarbon-contaminated groundwater.Molecular biotechnology · 2012Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mixed culture dechlorinating 1,2-dichloroethane (1,2-DCA) to ethene was enriched from groundwater that had been subjected to long-term contamination. In the metagenome of the enrichment, a 7-kb reductive dehalogenase (RD) gene cluster sequence was detected by inverse and direct PCR. The RD gene cluster had four open reading frames (ORF) showing 99% nucleotide identity with pceB, pceC, pceT, and orf1 of Dehalobacter restrictus strain DSMZ 9455(T), a bacterium able to dechlorinate chlorinated ethenes. However, dcaA, the ORF encoding the catalytic subunit, showed only 94% nucleotide and 90% amino acid identity with pceA of strain DSMZ 9455(T). Fifty-three percent of the amino acid differences were localized in two defined regions of the predicted protein. Exposure of the culture to 1,2-DCA and lactate increased the dcaA gene copy number by 2 log units, and under these conditions the dcaA and dcaB genes were actively transcribed. A very similar RD gene cluster with 98% identity in the dcaA gene sequence was identified in Desulfitobacterium dichloroeliminans strain DCA1, the only known isolate that selectively dechlorinates 1,2-DCA but not chlorinated ethenes. The dcaA gene of strain DCA1 possesses the same amino acid motifs as the new dcaA gene. Southern hybridization using total genomic DNA of strain DCA1 with dcaA gene-specific and dcaB- and pceB-targeting probes indicated the presence of two identical or highly similar dehalogenase gene clusters. In conclusion, these data suggest that the newly described RDs are specifically adapted to 1,2-DCA dechlorination.
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