ArticleApplied and environmental microbiology2007
The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter for the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2).
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Beyond Taxonomy: Fungal Nutraceutical Supplementation Modifies the Functional Potential of the Gut Microbiome in Colorectal Cancer.International journal of molecular sciences · 2026Trial
- Lon protease reprograms cellular physiology ofFrontiers in microbiology · 2026Article
- Streptomyces venezuelae uses secreted chitinases and a designated ABC transporter to support the competitive saprophytic catabolism of chitin.PLoS biology · 2025Article
- Article
- Genomic Exploration of a ChitinolyticCurrent issues in molecular biology · 2024Article
- Effects of the pleiotropic regulator DasR on lincomycin production in Streptomyces lincolnensis.Applied microbiology and biotechnology · 2024Article
- Regulation of Multidrug Efflux Pumps by TetR Family Transcriptional Repressor Negatively Affects Secondary Metabolism in Streptomyces coelicolor A3(2).Applied and environmental microbiology · 2023Article
- A DasA family sugar binding protein Ste2 links nutrient and oxidative stress to exopolysaccharides production in Streptomyces sp. 139.BMC microbiology · 2022Article
- Enhancement of bleomycin production in Streptomyces verticillus through global metabolic regulation of N-acetylglucosamine and assisted metabolic profiling analysis.Microbial cell factories · 2020Article
- Antibiotic Production and Antibiotic Resistance: The Two Sides of AbrB1/B2, a Two-Component System ofFrontiers in microbiology · 2020Article
- NgcEMicrobes and environments · 2018Article
- Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution.Applied microbiology and biotechnology · 2018Review
- Taxonomy, Physiology, and Natural Products of Actinobacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- A Phenotypic and Genotypic Analysis of the Antimicrobial Potential of CultivableFrontiers in microbiology · 2016Article
- Genome-wide analysis of in vivo binding of the master regulator DasR in Streptomyces coelicolor identifies novel non-canonical targets.PloS one · 2015Article
- Functional analysis of the N-acetylglucosamine metabolic genes of Streptomyces coelicolor and role in control of development and antibiotic production.Journal of bacteriology · 2012Article
- CebR as a master regulator for cellulose/cellooligosaccharide catabolism affects morphological development in Streptomyces griseus.Journal of bacteriology · 2009Article
- Lack of A-factor production induces the expression of nutrient scavenging and stress-related proteins in Streptomyces griseus.Molecular & cellular proteomics : MCP · 2009Article
- Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae.Journal of molecular biology · 2009Article
- Article
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12 authors.
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Abstract
N,N'-Diacetylchitobiose [(GlcNAc)(2)] induces the transcription of chitinase (chi) genes in Streptomyces coelicolor A3(2). Physiological studies showed that (GlcNAc)(2) addition triggered chi expression and increased the rate of (GlcNAc)(2) concentration decline in culture supernatants of mycelia already cultivated with (GlcNAc)(2), suggesting that (GlcNAc)(2) induced the synthesis of its own uptake system. Four open reading frames (SCO0531, SCO0914, SCO2946, and SCO5232) encoding putative sugar-binding proteins of ABC transporters were found in the genome by probing the 12-bp repeat sequence required for regulation of chi transcription. SCO5232, named dasA, showed transcriptional induction by (GlcNAc)(2) and N,N',N'''-triacetylchitotriose [(GlcNAc)(3)]. Surface plasmon resonance analysis showed that recombinant DasA protein exhibited the highest affinity for (GlcNAc)(2) (equilibrium dissociation constant [K(D)] = 3.22 x 10(-8)). In the dasA-null mutant, the rate of decline of the (GlcNAc)(2) concentration in the culture supernatant was about 25% of that in strain M145. The in vitro and in vivo data clearly demonstrated that dasA is involved in (GlcNAc)(2) uptake. Upstream and downstream of dasA, the transcriptional regulator gene (dasR) and two putative integral membrane protein genes (dasBC) are located in the opposite and same orientations, respectively. The expression of dasR and dasB, which seemed independent of dasA transcription, was also induced by (GlcNAc)(2) and (GlcNAc)(3).
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