ArticleBiophysical journal2007
Numerical calculations of single-cell electroporation with an electrolyte-filled capillary.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- 2D electrical admittance lattice model of biological cellular system for modeling electroporation.Biophysical journal · 2024Article
- Effect of input voltage frequency on the distribution of electrical stresses on the cell surface based on single-cell dielectrophoresis analysis.Scientific reports · 2020Article
- A Review on Electroporation-Based Intracellular Delivery.Molecules (Basel, Switzerland) · 2018Review
- Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.Nature communications · 2018Article
- Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Electroosmotic perfusion of tissue: sampling the extracellular space and quantitative assessment of membrane-bound enzyme activity in organotypic hippocampal slice cultures.Analytical and bioanalytical chemistry · 2014Review
- Single-cell juxtacellular transfection and recording technique.Pflugers Archiv : European journal of physiology · 2013Article
- Single-cell electroporation using a multifunctional pipette.Lab on a chip · 2012Article
- Control of the release of freely diffusing molecules in single-cell electroporation.Analytical chemistry · 2009Article
- Single-cell transfection by electroporation using an electrolyte/plasmid-filled capillary.Analytical chemistry · 2009Article
- Microscale electroporation: challenges and perspectives for clinical applications.Integrative biology : quantitative biosciences from nano to macro · 2009Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
An electric field is focused on one cell in single-cell electroporation. This enables selective electroporation treatment of the targeted cell without affecting its neighbors. While factors that lead to membrane permeation are the same as in bulk electroporation, quantitative description of the single-cell experiments is more complicated. This is due to the fact that the potential distribution cannot be solved analytically. We present single-cell electroporation with an electrolyte-filled capillary modeled with a finite element method. Potential is calculated in the capillary, the solution surrounding the cell, and the cell. The model enables calculation of the transmembrane potential and the fraction of the cell membrane that is above the critical electroporation potential. Electroporation at several cell-to-tip distances of human lung carcinoma cells (A549) stained with ThioGlo-1 demonstrated membrane permeation at distances shorter than approximately 7.0 microm. This agrees well with the model's prediction that a critical transmembrane potential of 250 mV is achieved when the capillary is approximately 6.5 microm or closer to the cell. Simulations predict that at short cell-to-tip distances, the transmembrane potential increases significantly while the total area of the cell above the critical potential increases only moderately.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.