ArticleCell2007
Mechanism of actin network attachment to moving membranes: barbed end capture by N-WASP WH2 domains.
Article in Cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
108 citing papers in PubMed.
- When two worlds collide: actin dynamics on endomembranes regulates membrane trafficking.Current opinion in cell biology · 2025Review
- Membrane-associated polymerases deliver most of the actin subunits to a lamellipodial network.bioRxiv : the preprint server for biology · 2025Article
- The actin filament pointed-end depolymerase Srv2/CAP depolymerizes barbed ends, displaces capping protein, and promotes formin processivity.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A unified purification method for actin-binding proteins using a TEV-cleavable His-Strep-tag.MethodsX · 2024Article
- Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion.Nature cell biology · 2024Article
- An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis.Nature communications · 2024Article
- Bsp1, a fungal CPI motif protein, regulates actin filament capping in endocytosis and cytokinesis.Molecular biology of the cell · 2024Article
- Polymerization force-regulated actin filament-Arp2/3 complex interaction dominates self-adaptive cell migrations.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Article
- Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration.Nature cell biology · 2021Article
- Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.Communications biology · 2021Article
- A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks.Nature communications · 2021Article
- The Mechanics and Thermodynamics of Tubule Formation in Biological Membranes.The Journal of membrane biology · 2021Review
- Phosphorylation of the WH2 domain in yeast Las17/WASP regulates G-actin binding and protein function during endocytosis.Scientific reports · 2021Article
- Evolutionarily related small viral fusogens hijack distinct but modular actin nucleation pathways to drive cell-cell fusion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge.Molecular biology of the cell · 2020Article
- Article
- Spire stimulates nucleation by Cappuccino and binds both ends of actin filaments.Molecular biology of the cell · 2020Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Actin filament networks exert protrusive and attachment forces on membranes and thereby drive membrane deformation and movement. Here, we show that N-WASP WH2 domains play a previously unanticipated role in vesicle movement by transiently attaching actin filament barbed ends to the membrane. To dissect the attachment mechanism, we reconstituted the propulsive motility of lipid-coated glass beads, using purified soluble proteins. N-WASP WH2 mutants assembled actin comet tails and initiated movement, but the comet tails catastrophically detached from the membrane. When presented on the surface of a lipid-coated bead, WH2 domains were sufficient to maintain comet tail attachment. In v-Src-transformed fibroblasts, N-WASP WH2 mutants were severely defective in the formation of circular podosome arrays. In addition to creating an attachment force, interactions between WH2 domains and barbed ends may locally amplify signals for dendritic actin nucleation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.