ArticleJournal of the American Society for Mass Spectrometry2007
Liquid chromatography electron capture dissociation tandem mass spectrometry (LC-ECD-MS/MS) versus liquid chromatography collision-induced dissociation tandem mass spectrometry (LC-CID-MS/MS) for the identification of proteins.
Article in Journal of the American Society for Mass Spectrometry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Predicting protein post-translational modifications using meta-analysis of proteome scale data sets.Molecular & cellular proteomics : MCP · 2009Pooled it
- VUV Photodissociation Induced by a Deuterium Lamp in an Ion Trap.Journal of the American Society for Mass Spectrometry · 2019Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Conversion of 3-nitrotyrosine to 3-aminotyrosine residues facilitates mapping of tyrosine nitration in proteins by electrospray ionization-tandem mass spectrometry using electron capture dissociation.Journal of mass spectrometry : JMS · 2012Article
- Using electron induced dissociation (EID) on an LC time-scale to characterize a mixture of analogous small organic molecules.Journal of the American Society for Mass Spectrometry · 2012Article
- Sequential abundant ion fragmentation analysis (SAIFA): an alternative approach for phosphopeptide identification using an ion trap mass spectrometer.Analytical biochemistry · 2011Article
- Novel glycosylation sites localized in Campylobacter jejuni flagellin FlaA by liquid chromatography electron capture dissociation tandem mass spectrometry.Journal of proteome research · 2011Article
- Mass spectrometry in chronic kidney disease research.Advances in chronic kidney disease · 2010Review
- Clustered O-glycans of IgA1: defining macro- and microheterogeneity by use of electron capture/transfer dissociation.Molecular & cellular proteomics : MCP · 2010Article
- Characterization of an Ion Mobility-Multiplexed Collision Induced Dissociation-Tandem Time-of-Flight Mass Spectrometry Approach.International journal of mass spectrometry · 2010Article
- Identification of carbonylation sites in apomyoglobin after exposure to 4-hydroxy-2-nonenal by solid-phase enrichment and liquid chromatography-electrospray ionization tandem mass spectrometry.Journal of mass spectrometry : JMS · 2010Article
- Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry.Molecular & cellular proteomics : MCP · 2009Article
- Analysis of the trypanosome flagellar proteome using a combined electron transfer/collisionally activated dissociation strategy.Journal of the American Society for Mass Spectrometry · 2009Article
- The effect of phosphorylation on the electron capture dissociation of peptide ions.Journal of the American Society for Mass Spectrometry · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Electron capture dissociation (ECD) offers many advantages over the more traditional fragmentation techniques for the analysis of peptides and proteins, although the question remains: How suitable is ECD for incorporation within proteomic strategies for the identification of proteins? Here, we compare LC-ECD-MS/MS and LC-CID-MS/MS as techniques for the identification of proteins. Experiments were performed on a hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer. Replicate analyses of a six-protein (bovine serum albumin, apo-transferrin, lysozyme, cytochrome c, alcohol dehydrogenase, and beta-galactosidase) tryptic digest were performed and the results analyzed on the basis of overall protein sequence coverage and sequence tag lengths within individual peptides. The results show that although protein coverage was lower for LC-ECD-MS/MS than for LC-CID-MS/MS, LC-ECD-MS/MS resulted in longer peptide sequence tags, providing greater confidence in protein assignment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.