ArticleMarine pollution bulletin2007
Luminex detection of fecal indicators in river samples, marine recreational water, and beach sand.
Article in Marine pollution bulletin, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Etiology and severity of diarrheal diseases in infants at the semiarid region of Brazil: A case-control study.PLoS neglected tropical diseases · 2019Article
- Antimicrobial Susceptibility of Staphylococcus aureus Isolated from Recreational Waters and Beach Sand in Eastern Cape Province of South Africa.International journal of environmental research and public health · 2017Article
- Ex situ study of Enterococcus faecalis survival in the recreational waters of the southern coast of the Caspian Sea.Iranian journal of microbiology · 2016Article
- Water quality, weather and environmental factors associated with fecal indicator organism density in beach sand at two recreational marine beaches.The Science of the total environment · 2014Article
- Enterococci in the environment.Microbiology and molecular biology reviews : MMBR · 2012Review
- Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice.The Journal of infectious diseases · 2012Article
- Simultaneous detection of six diarrhea-causing bacterial pathogens with an in-house PCR-luminex assay.Journal of clinical microbiology · 2012Article
- Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres.Journal of clinical microbiology · 2009Article
- Pepper mild mottle virus as an indicator of fecal pollution.Applied and environmental microbiology · 2009Article
- Faecal indicator bacteria enumeration in beach sand: a comparison study of extraction methods in medium to coarse sands.Journal of applied microbiology · 2009Article
- A Preliminary Investigation of Fecal Indicator Bacteria, Human Pathogens, and Source Tracking Markers in Beach Water and Sand.Environment research journal · 2009Article
- The coastal environment and human health: microbial indicators, pathogens, sentinels and reservoirs.Environmental health : a global access science source · 2008Review
- Food microbial pathogen detection and analysis using DNA microarray technologies.Foodborne pathogens and disease · 2008Article
- Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology.Journal of clinical microbiology · 2007Article
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Research to understand and remediate coastal pollution is moving toward a multitiered approach in which traditional enumeration of fecal indicators is accompanied by molecular analysis of a variety of targets. Technology that rapidly detects multiple microbial contaminants would benefit from such an approach. The Luminex 100 system is a suspension array that assays multiple analytes rapidly in a single well of a microtiter plate. The ability of the system to simultaneously detect multiple fecal indicating bacteria in environmental samples was tested. Primer/probe sets were designed to simultaneously detect the following fecal indicators: the Bacteroides fragilis group, Enterococcus spp., Escherichia coli and Shigella spp., Bacteroides distasonis, and Ent. faecalis. Specificity and sensitivity of the Luminex probes was tested against laboratory cultures. In addition, sequencing, culture plate testing, and specificity testing with environmental isolates were steps taken to validate the function of the assay with environmental samples. Luminex response to cultures and to environmental samples was consistent with sequencing results, suggesting that the technology has the potential to simultaneously detect multiple targets for coastal water quality applications, particularly as progress is made to efficiently extract DNA from water and sediment matrices.
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Registered trials
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