ArticleExperimental gerontology2007
Alteration of Drosophila life span using conditional, tissue-specific expression of transgenes triggered by doxycyline or RU486/Mifepristone.
Article in Experimental gerontology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 90 citations in OpenAlex.
- Pharmacological protection from a sugar-rich diet through inhibition of Ras/MAPK signaling.iScience · 2026Article
- Suppression rather than activation of the integrated stress response (GCN2-ATF4) pathway extends lifespan in the fly.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
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- Conditional Inhibition of Eip75B Eliminates the Effects of Mating and Mifepristone on Lifespan in FemaleCells · 2024Article
- The importance of host physical niches for the stability of gut microbiome composition.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- A tailed mirtron promotes longevity in Drosophila.Nucleic acids research · 2024Article
- An Adapted GeneSwitch Toolkit for Comparable Cellular and Animal Models: A Proof of Concept in Modeling Charcot-Marie-Tooth Neuropathy.International journal of molecular sciences · 2023Article
- Use of Farnesyl Transferase Inhibitors in an Ageing Model inJournal of developmental biology · 2023Article
- Mifepristone Increases Life Span in FemaleFrontiers in aging · 2022Article
- Review
- Mifepristone (RU486) inhibits dietary lipid digestion by antagonizing the role of glucocorticoid receptor on lipase transcription.iScience · 2021Article
- Regulation ofFrontiers in neuroscience · 2021Article
- Mifepristone Increases Life Span of Virgin FemaleFrontiers in genetics · 2021Article
- Fine-tuning autophagy maximises lifespan and is associated with changes in mitochondrial gene expression in Drosophila.PLoS genetics · 2020Article
- Independent glial subtypes delay development and extend healthy lifespan upon reduced insulin-PI3K signalling.BMC biology · 2020Article
- GWAS for Lifespan and Decline in Climbing Ability in Flies upon Dietary Restriction Reveal decima as a Mediator of Insulin-like Peptide Production.Current biology : CB · 2020Article
- The neuronal receptor tyrosine kinase Alk is a target for longevity.Aging cell · 2020Article
- Partial Inhibition of RNA Polymerase I Promotes Animal Health and Longevity.Cell reports · 2020Article
- Analysis of Drosophila melanogaster Lifespan.Methods in molecular biology (Clifton, N.J.) · 2020Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The conditional systems Tet-on and Geneswitch were compared and optimized for the tissue-specific expression of transgenes and manipulation of life span in adult Drosophila. Two versions of Tet-on system reverse-tetracycline-Trans-Activator (rtTA) were compared: the original rtTA, and rtTAM2-alt containing mutations designed to optimize regulation and expression. The rtTAM2-alt version gave less leaky expression of target constructs in the absence of doxycyline, however the absolute level of expression that could be achieved was less than that produced by rtTA, in contrast to a previous report. Existing UAS-rtTAM2-alt insertions were re-balanced, and combined with several tissue-general and tissue-specific GAL4 driver lines to yield tissue-specific, doxycyline-inducible transgene expression over three orders of magnitude. The Geneswitch (GS) system also had low background, but the absolute level of expression was low relative to Tet-on. Consequently, actin5C-GS multi-insert chromosomes were generated and higher-level expression was achieved without increased background. Moderate level over-expression of MnSOD has beneficial effects on life span. Here high-level over-expression of MnSOD was found to have toxic effects. In contrast, motor-neuron-specific over-expression of MnSOD had no detectable effect on life span. The results suggest that motor-neuron tissue is not the essential tissue for either MnSOD induced longevity or toxicity in adult males.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.