ArticleParasitology research2007
Identification of differentially expressed genes in female Culex pipiens pallens.
Article in Parasitology research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Salivary gland proteome of the human malaria vector, Anopheles campestris-like (Diptera: Culicidae).Parasitology research · 2013Article
- Cloning, expression, and characterization of salivary apyrase from Aedes albopictus.Parasitology research · 2012Article
- Differentially expressed genes between female and male adult Anopheles anthropophagus.Parasitology research · 2009Article
- Analysis and comparison of a set of expressed sequence tags of the parthenogenetic water flea Daphnia carinata.Molecular genetics and genomics : MGG · 2009Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Culex pipiens pallens is the mosquito vector of a number of human pathogens such as Wuchereria bancrofti, Brugia malayi, and epidemic encephalitis B virus. Female C. pipiens pallens play an important role in transmitting pathogens by sucking blood, which is essential for reproduction. In the present study, a subtractive cDNA library for female C. pipiens pallens was constructed by the suppression subtractive hybridization (SSH) technique and then 100 clones from the female SSH library were sequenced and analyzed. Female-differentially expressed genes in C. pipiens pallens were screened using semiquantitative RT-PCR. The full-length cDNA of an EST sequence (fs68) that was specifically expressed in female C. pipiens pallens was characterized by 3' and 5' rapid amplification of cDNA ends (RACE). The characteristics of the female-specific gene were further analyzed using bioinformatics and Northern blot. It was shown that the female-specific gene was a previously uncharacterized gene and may encode a salivary peptide. This putative salivary peptide could be a very important molecule in the blood feeding of female C. pipiens pallens.
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