ArticleThe Journal of physiology2007
Plasma membrane delivery, endocytosis and turnover of transcobalamin receptor in polarized human intestinal epithelial cells.
Article in The Journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Evidence for an Aneugenic Mechanism of Action for Micronucleus Induction by Black Cohosh Extract.Environmental and molecular mutagenesis · 2019Article
- Kinetic analysis of transcellular passage of the cobalamin-transcobalamin complex in Caco-2 monolayers.Molecular biology of the cell · 2019Article
- Transcellular transport of cobalamin in aortic endothelial cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Black cohosh extracts and powders induce micronuclei, a biomarker of genetic damage, in human cells.Environmental and molecular mutagenesis · 2018Article
- Parallel mRNA, proteomics and miRNA expression analysis in cell line models of the intestine.World journal of gastroenterology · 2017Article
- Absorption and blood/cellular transport of folate and cobalamin: Pharmacokinetic and physiological considerations.Biochimie · 2016Review
- Article
- Vitamin B-12 and Perinatal Health.Advances in nutrition (Bethesda, Md.) · 2015Review
- Pancreatic α-Amylase Controls Glucose Assimilation by Duodenal Retrieval through N-Glycan-specific Binding, Endocytosis, and Degradation.The Journal of biological chemistry · 2015Article
- Expression and function of the lipocalin-2 (24p3/NGAL) receptor in rodent and human intestinal epithelia.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells that are metabolically active and in a high degree of differentiation and proliferation require cobalamin (Cbl: vitamin B(12)) and they obtain it from the circulation bound to transcobalamin (TC) via the transcobalamin receptor (TC-R). This study has investigated the plasma membrane dynamics of TC-R expression in polarized human intestinal epithelial Caco-2 cells using techniques of pulse-chase labelling, domain-specific biotinylation and cell fractionation. Endogenously synthesized TC-R turned over with a half-life (T(1/2)) of 8 h following its delivery to the basolateral plasma membrane (BLM). The T(1/2) of BLM delivery was 15 min and TC-R delivered to the BLM was endocytosed and subsequently degraded by leupeptin-sensitive proteases. However, about 15% of TC-R endocytosed from the BLM was transcytosed (T(1/2), 45 min) to the apical membranes (BBM) where it underwent endocytosis and was degraded. TC-R delivery to both BLM and BBM was inhibited by Brefeldin A and tunicamycin, but not by wortmannin or leupeptin. Colchicine inhibited TC-R delivery to BBM, but not BLM. At steady state, apical TC-R was associated with megalin and both these proteins were enriched in an intracellular compartment which also contained Rab5 and transferrin receptor. These results indicate that following rapid delivery to both plasma membrane domains of Caco-2 cells, TC-R undergoes constitutive endocytosis and degradation by leupeptin-sensitive proteases. TC-R expressed in apical BBM complexes with megalin during its transcytosis from the BLM.
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Registered trials
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