ArticleMolecular biology of the cell2007
A Ser/Thr kinase required for membrane-associated assembly of the major sperm protein motility apparatus in the amoeboid sperm of Ascaris.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Released ATP Mediates Spermatozoa Chemotaxis Promoted by Uterus-Derived Factor (UDF) inInternational journal of molecular sciences · 2022Article
- Extending the molecular clutch beyond actin-based cell motility.New journal of physics · 2014Article
- Article
- Cytosolic Ca(2+) as a multifunctional modulator is required for spermiogenesis in Ascaris suum.Protein & cell · 2013Article
- Spermatogenesis.Advances in experimental medicine and biology · 2013Review
- Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans.Cell calcium · 2013Review
- Transformation: how do nematode sperm become activated and crawl?Protein & cell · 2012Review
- Nematode sperm maturation triggered by protease involves sperm-secreted serine protease inhibitor (Serpin).Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.Genetics · 2012Article
- Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad.BMC genomics · 2011Article
- Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.International journal of biochemistry and molecular biology · 2011Article
- New insights into the mechanism of fertilization in nematodes.International review of cell and molecular biology · 2011Review
- Spermatogenesis-defective (spe) mutants of the nematode Caenorhabditis elegans provide clues to solve the puzzle of male germline functions during reproduction.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
- Biochemical mechanisms for regulating protrusion by nematode major sperm protein.Biophysical journal · 2009Article
- Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Leading edge protrusion in the amoeboid sperm of Ascaris suum is driven by the localized assembly of the major sperm protein (MSP) cytoskeleton in the same way that actin assembly powers protrusion in other types of crawling cell. Reconstitution of this process in vitro led to the identification of two accessory proteins required for MSP polymerization: an integral membrane phosphoprotein, MSP polymerization-organizing protein (MPOP), and a cytosolic component, MSP fiber protein 2 (MFP2). Here, we identify and characterize a 34-kDa cytosolic protein, MSP polymerization-activating kinase (MPAK) that links the activities of MPOP and MFP2. Depletion/add-back assays of sperm extracts showed that MPAK, which is a member of the casein kinase 1 family of Ser/Thr protein kinases, is required for motility. MPOP and MPAK comigrated by native gel electrophoresis, coimmunoprecipitated, and colocalized by immunofluorescence, indicating that MPOP binds to and recruits MPAK to the membrane surface. MPAK, in turn, phosphorylated MFP2 on threonine residues, resulting in incorporation of MFP2 into the cytoskeleton. Beads coated with MPAK assembled a surrounding cloud of MSP filaments when incubated in MPAK-depleted sperm extract, but only when supplemented with detergent-solubilized MPOP. Our results suggest that interactions involving MPOP, MPAK, and MFP2 focus MSP polymerization to the plasma membrane at the leading edge of the cell thereby generating protrusion and minimizing nonproductive filament formation elsewhere.
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