ArticleMolecular biology of the cell2007
Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Anti-centrosome antibodies in breast cancer are the expression of autoimmunity.Immunologic research · 2014Article
- Cell type-specific expression of SEPT3-homology subgroup members controls the subunit number of heteromeric septin complexes.Molecular biology of the cell · 2014Article
- cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin phosphorylation for the maintenance of interphase microtubules during osmotic stress.The Journal of biological chemistry · 2014Article
- Stathmin and microtubules regulate mitotic entry in HeLa cells by controlling activation of both Aurora kinase A and Plk1.Molecular biology of the cell · 2013Article
- Septins 2, 7 and 9 and MAP4 colocalize along the axoneme in the primary cilium and control ciliary length.Journal of cell science · 2013Article
- The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila.PloS one · 2013Article
- Mammalian SEPT9 isoforms direct microtubule-dependent arrangements of septin core heteromers.Molecular biology of the cell · 2012Article
- The microtubule cytoskeleton is required for a G2 cell cycle delay in cancer cells lacking stathmin and p53.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells.Molecular biology of the cell · 2011Article
- Deciphering the rules governing assembly order of mammalian septin complexes.Molecular biology of the cell · 2011Article
- 2D difference gel electrophoresis analysis of different time points during the course of neoplastic transformation of human mammary epithelial cells.Journal of proteome research · 2011Article
- Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.Cellular and molecular life sciences : CMLS · 2009Review
- Stathmin regulates centrosomal nucleation of microtubules and tubulin dimer/polymer partitioning.Molecular biology of the cell · 2009Article
- Article
- Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.Molecular biology of the cell · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microtubule cytoskeleton is differentially regulated by a diverse array of proteins during interphase and mitosis. Op18/stathmin (Op18) and microtubule-associated protein (MAP)4 have been ascribed opposite general microtubule-directed activities, namely, microtubule destabilization and stabilization, respectively, both of which can be inhibited by phosphorylation. Here, using three human cell models, we depleted cells of Op18 and/or MAP4 by expression of interfering hairpin RNAs and we analyzed the resulting phenotypes. We found that the endogenous levels of Op18 and MAP4 have opposite and counteractive activities that largely govern the partitioning of tubulin dimers in the microtubule array at interphase. Op18 and MAP4 were also found to be the downstream targets of Ca(2+)- and calmodulin-dependent protein kinase IV and PAR-1/MARK2 kinase, respectively, that control the demonstrated counteractive phosphorylation-mediated regulation of tubulin dimer partitioning. Furthermore, to address mechanisms regulating microtubule polymerization in response to cell signals, we developed a system for inducible gene product replacement. This approach revealed that site-specific phosphorylation of Op18 is both necessary and sufficient for polymerization of microtubules in response to the multifaceted signaling event of stimulation of the T cell antigen receptor complex, which activates several signal transduction pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.