ArticleBMC microbiology2007
Characterization of Escherichia coli MG1655 grown in a low-shear modeled microgravity environment.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 81 citations in OpenAlex.
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- Microbiology of human spaceflight: microbial responses to mechanical forces that impact health and habitat sustainability.Microbiology and molecular biology reviews : MMBR · 2024Review
- Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity.NPJ microgravity · 2024Article
- Effect of spaceflight on the phenotype and proteome ofOpen life sciences · 2023Article
- Phenotypic, genomic, and transcriptomic changes in an Acinetobacter baumannii strain after spaceflight in China's Tiangong-2 space laboratory.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Adaptation to simulated microgravity in Streptococcus mutans.NPJ microgravity · 2022Article
- Growth Behavior and Transcriptome Profile Analysis ofPolish journal of microbiology · 2022Article
- Combined Impact of Magnetic Force and Spaceflight Conditions onInternational journal of molecular sciences · 2022Article
- Changes in Higher-Order Chromosomal Structure ofFrontiers in microbiology · 2022Article
- Spaceflight Analogue Culture Enhances the Host-Pathogen Interaction BetweenFrontiers in cellular and infection microbiology · 2022Article
- Advances in space microbiology.iScience · 2021Review
- The influence of spaceflight and simulated microgravity on bacterial motility and chemotaxis.NPJ microgravity · 2021Review
- Effects of Simulated Microgravity on the Physiology ofFrontiers in microbiology · 2021Article
- Transcriptional Profiling of the ProbioticInternational journal of molecular sciences · 2020Article
- Omics Analysis Reveals the Mechanism of Enhanced Recombinant Protein Production Under Simulated Microgravity.Frontiers in bioengineering and biotechnology · 2020Article
- Clinostat Rotation Affects Metabolite Transportation and Increases Organic Acid Production byApplied and environmental microbiology · 2019Article
- Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExtra-cellular shear force is an important environmental parameter that is significant both medically and in the space environment. Escherichia coli cells grown in a low-shear modeled microgravity (LSMMG) environment produced in a high aspect rotating vessel (HARV) were subjected to transcriptional and physiological analysis.
resultsAerobic LSMMG cultures were grown in rich (LB) and minimal (MOPS + glucose) medium with a normal gravity vector HARV control. Reproducible changes in transcription were seen, but no specific LSMMG responsive genes were identified. Instead, absence of shear and a randomized gravity vector appears to cause local extra-cellular environmental changes, which elicit reproducible cellular responses. In minimal media, the majority of the significantly up- or down-regulated genes of known function were associated with the cell envelope. In rich medium, most LSMMG down-regulated genes were involved in translation. No observable changes in post-culture stress responses and antibiotic sensitivity were seen in cells immediately after exposure to LSMMG. Comparison with earlier studies of Salmonella enterica serovar Typhimurium conducted under similar growth conditions, revealed essentially no similarity in the genes that were significantly up- or down-regulated.
conclusionComparison of these results to previous studies suggests that different organisms may dramatically differ in their responses to medically significant low-shear and space environments. Depending on their specific response, some organisms, such as Salmonella, may become preadapted in a manner that predisposes them to increased virulence.
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