ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2007
Flow analysis and sorting of microchromosomes (<3 Mb).
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Hi-C scaffolded short- and long-read genome assemblies of the California sea lion are broadly consistent for syntenic inference across 45 million years of evolution.Molecular ecology resources · 2021Article
- Chromosome-level genome assembly for giant panda provides novel insights into Carnivora chromosome evolution.Genome biology · 2019Article
- Chromosome Analysis Using Benchtop Flow Analysers and High Speed Cell Sorters.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Article
- Human Y chromosome base-substitution mutation rate measured by direct sequencing in a deep-rooting pedigree.Current biology : CB · 2009Article
- Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.Nature protocols · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe analysis and isolation of high numbers of chromosomes smaller than 3 Mb in size (microchromosomes) with good purity is dependent primarily on the detection sensitivity of the flow cytometer and the precision of the sort unit. The aim of this study was to investigate the capability of using a conventional flow cytometer for the detection and sorting at high purity microchromosomes with an estimated size of 2.7 Mb.
methodsChromosomes were isolated from a human cell line containing a pair of X-derived microchromosomes, using a modified polyamine isolation buffer. The chromosome preparation was labeled with Hoechst and Chromomycin and analyzed and purified using a MoFlo sorter (DAKO) configured for high-speed sorting. The purity of the flow-sorted microchromosomes was assessed by reverse chromosome painting.
resultsImproved resolution of the peak of microchromosomes in a bivariate plot of Hoechst versus Chromomycin fluorescence was obtainable after discriminating clumps and debris based on gating data within a FSC versus pulse width plot.
conclusionsChromosomes of smaller size, less than 3 Mb, can be detected with high resolution and flow-sorted with high purity using a conventional flow sorter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.