ArticleMarine biotechnology (New York, N.Y.)
Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Establishment, identification, and transcriptome analysis of a Sertoli cell line from ovoviviparous black rockfish Sebastes schlegelii.Fish physiology and biochemistry · 2025Article
- Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.Fish physiology and biochemistry · 2018Article
- Dopamine regulates renal osmoregulation during hyposaline stress via DRD1 in the spotted scat (Scatophagus argus).Scientific reports · 2016Article
- RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax.Cytotechnology · 2014Article
- Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.Fish physiology and biochemistry · 2013Article
- Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2012Article
- Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).Fish physiology and biochemistry · 2012Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
- Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2010Article
- Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus).Fish physiology and biochemistry · 2010Article
- Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus.Cytotechnology · 2010Article
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Authors and funding
4 authors.
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Abstract
A marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25 degrees C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with 2n = 8st + 40t. A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (10(8.5) TCID(50) ml(-1)), while the viral titer of frog Rana grylio virus 9807 (RGV(9807)) reached 10(3.5) TCID(50) ml(-1). The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.