ArticleBlood2007
Roles of RabGEF1/Rabex-5 domains in regulating Fc epsilon RI surface expression and Fc epsilon RI-dependent responses in mast cells.
Article in Blood, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.Frontiers in immunology · 2018Article
- Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis.The Journal of clinical investigation · 2016Article
- An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria.Microbial cell factories · 2016Article
- Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Enhanced Purification of Ubiquitinated Proteins by Engineered Tandem Hybrid Ubiquitin-binding Domains (ThUBDs).Molecular & cellular proteomics : MCP · 2016Article
- The CD20 homologue MS4A4 directs trafficking of KIT toward clathrin-independent endocytosis pathways and thus regulates receptor signaling and recycling.Molecular biology of the cell · 2015Article
- RabGEF1/Rabex-5 Regulates TrkA-Mediated Neurite Outgrowth and NMDA-Induced Signaling Activation in NGF-Differentiated PC12 Cells.PloS one · 2015Article
- Rapid desensitization induces internalization of antigen-specific IgE on mouse mast cells.The Journal of allergy and clinical immunology · 2013Article
- Spatiotemporal regulation of the ubiquitinated cargo-binding activity of Rabex-5 in the endocytic pathway.The Journal of biological chemistry · 2012Article
- RIN3 is a negative regulator of mast cell responses to SCF.PloS one · 2012Article
- Rab22 controls NGF signaling and neurite outgrowth in PC12 cells.Molecular biology of the cell · 2011Article
- Immunoglobulin E receptor signaling and asthma.The Journal of biological chemistry · 2011Review
- Particulate allergens potentiate allergic asthma in mice through sustained IgE-mediated mast cell activation.The Journal of clinical investigation · 2011Article
- Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.The American journal of pathology · 2010Article
- Feedback regulation of Ras signaling by Rabex-5-mediated ubiquitination.Current biology : CB · 2010Article
- Delayed onset of positive feedback activation of Rab5 by Rabex-5 and Rabaptin-5 in endocytosis.PloS one · 2010Article
- Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis.Molecular biology of the cell · 2009Article
- Functional determinants of ras interference 1 mutants required for their inhbitory activity on endocytosis.Experimental cell research · 2009Article
- Contributions of F-BAR and SH2 domains of Fes protein tyrosine kinase for coupling to the FcepsilonRI pathway in mast cells.Molecular and cellular biology · 2009Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
RabGEF1/Rabex-5, a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator, Rab5, contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain. To investigate the importance of these domains in regulating receptor internalization and cell activation, we lentivirally delivered RabGEF1 mutants into RabGEF1-deficient (-/-) mast cells and examined Fc epsilon RI-dependent responses. Wild-type RabGEF1 expression corrected phenotypic abnormalities in -/- mast cells, including decreased basal Fc epsilon RI expression, slowed Fc epsilon RI internalization, elevated IgE + Ag-induced degranulation and IL-6 production, and the decreased ability of -/- cytosol to support endosome fusion. We showed that RabGEF1's ZnF domain has ubiquitin ligase activity. Moreover, the coiled coil domain of RabGEF1 is required for Rabaptin-5 binding and for maintaining basal levels of Rabaptin-5 and surface Fc epsilon RI. However, mutants lacking either of these domains normalized phenotypic abnormalities in IgE + antigen-activated -/- mast cells. By contrast, correction of these -/- phenotypes required a functional Vps9 domain. Thus, Fc epsilon RI-mediated mast cell functional activation is dependent on RabGEF1's GEF activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.