ArticleThe Journal of cell biology1992
A normally masked nuclear matrix antigen that appears at mitosis on cytoskeleton filaments adjoining chromosomes, centrioles, and midbodies.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- The host protein CLUH participates in the subnuclear transport of influenza virus ribonucleoprotein complexes.Nature microbiology · 2016Article
- Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns.Methods (San Diego, Calif.) · 2016Article
- Article
- Influenza virus ribonucleoprotein complexes gain preferential access to cellular export machinery through chromatin targeting.PLoS pathogens · 2011Article
- Article
- Nuclear localization of flavivirus RNA synthesis in infected cells.Journal of virology · 2006Article
- Mechanogenomic control of DNA exposure and sequestration.The American journal of pathology · 2005Review
- Chromatin organization measured by AluI restriction enzyme changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton.The American journal of pathology · 2005Article
- Altered localization of retinoid X receptor alpha coincides with loss of retinoid responsiveness in human breast cancer MDA-MB-231 cells.Molecular and cellular biology · 2004Article
- The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly.The Journal of cell biology · 1999Article
- Detergent-salt resistance of LAP2alpha in interphase nuclei and phosphorylation-dependent association with chromosomes early in nuclear assembly implies functions in nuclear structure dynamics.The EMBO journal · 1998Article
- A coactivator of pre-mRNA splicing.Genes & development · 1998Article
- Cell cycle changes in A-type lamin associations detected in human dermal fibroblasts using monoclonal antibodies.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1997Article
- Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Protein 4.1 is a component of the nuclear matrix of mammalian cells.The Biochemical journal · 1995Article
- The nuclear matrix phosphoprotein p255 associates with splicing complexes as part of the [U4/U6.U5] tri-snRNP particle.Nucleic acids research · 1995Article
- Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains.The Journal of cell biology · 1995Article
- Association of nuclear matrix antigens with exon-containing splicing complexes.The Journal of cell biology · 1994Article
- The B1C8 protein is in the dense assemblies of the nuclear matrix and relocates to the spindle and pericentriolar filaments at mitosis.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- The product of the UL31 gene of herpes simplex virus 1 is a nuclear phosphoprotein which partitions with the nuclear matrix.Journal of virology · 1993Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
mAbs were generated against HeLa nuclear matrix proteins and one, HIB2, which selectively stained mitotic cells, was selected for further study. Western blot analysis showed H1B2 antibody detected a protein of 240 kD in the nuclear matrix fractions. The H1B2 antigen was completely masked in immunofluorescently stained interphase cells. However, removing chromatin with DNase I digestion and 0.25 M ammonium sulfate extraction exposed the protein epitope. The resulting fluorescence pattern was bright, highly punctate, and entirely nuclear. Further extraction of the nuclear matrix with 2 M NaCl uncovers an underlying, anastomosing network of 9-13 nm core filaments. Most of the H1B2 antigen was retained in the fibrogranular masses enmeshed in the core filament network and not in the filaments themselves. The H1B2 antigen showed remarkable behavior at mitosis. As cells approached prophase the antigen became unmasked to immunofluorescent staining without the removal of chromatin. First appearing as a bright spot, the antibody staining spread through the nucleus finally concentrating in the region around the condensed chromosomes. The antibody also brightly stained the spindle poles and, more weakly, in a punctate pattern in the cytoskeleton around the spindle. As the chromosomes separated at anaphase, H1B2 remained with the separating daughter sets of chromosomes. The H1B2 antigen returned to the reforming nucleus at telophase, but left a bright staining region in the midbody. Immunoelectron microscopy of resinless sections showed that, in the mitotic cell, the H1B2 antibody did not stain chromosomes and centrioles themselves, but decorated a fibrogranular network surrounding and connected to the chromosomes and a fibrogranular structure surrounding the centriole.
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